Recombinant Bacillus subtilis for increasing yield of menaquinone 7 and application thereof

A recombinant Bacillus subtilis for increasing the yield of menaquinone 7, wherein Bacillus subtilis 168 is taken as an original strain;

• natural promoters of a menaquinone-specific isochorismate synthase gene menF and a dihydroxynaphthoic acid synthetase gene menB on a chromosome are replaced with P43 promoters;
• natural promoters of an O-succinylbenzoic acid-CoA ligase gene menE and a transketolase gene tkt on the chromosome are replaced with Phbs promoters;
• expression of an exogenous isochorismate synthase gene entC and an exogenous phosphoenolpyruvate synthetase gene ppsA on the chromosome are enhanced with P43 promoters, and a phosphotransferase system (PTS) glucose-specific enzyme IICBA component gene ptsG on the chromosome is knocked out;
• the sequence of the P43 promoter is shown in SEQ ID NO:
• 3;
• and the sequence of the Phbs promoter is shown in SEQ ID NO:
• 5.