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US 12,037,356 B2Granted
Methods of preparing steviol glycosides and uses of the same
AssigneePureCircle USA Inc.
InventorsAvetik Markosyan, Siew Yin Chow, Khairul Nizam Bin Nawi
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Abstract
Methods of preparing steviol glycosides, including Rebaudioside D, Rebaudioside E, Rebaudioside M, Rebaudioside N and Rebaudioside O are provided herein. Sweetener and sweetened consumables containing Rebaudioside D, Rebaudioside E, Rebaudioside M, Rebaudioside N and Rebaudioside O are also provided herein.
Claims13
A method for preparing a steviol glycosides composition comprising the steps of:
- (a) passing an initial mixture of steviol glycosides further comprising an initial solvent through a column packed with a polymer resin wherein the steviol glycosides are retained weakly in the column;
- and (b) using a second solvent, which is different than the initial solvent used in step (a) to collect a fraction with a high content of Reb D, Reb E, Reb M, Reb N and Reb O, to provide a solution with high content of Reb D, Reb E, Reb M, Reb N and Reb O, wherein the Reb DEMNO/T13SG ratio in the solution is higher than the Reb DEMNO/T13SG ratio of the initial mixture of steviol glycosides.
Dependencies are inferred from the claim text.