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US 12,035,726 B2Granted
Method for producing a cheese with reduced amount of galactose
AssigneeChr. Hansen A/S
InventorsThomas Janzen, Ditte Ellegaard Christiansen, Veronique Jactat, Kasper Rosenkvist, Charlotte Elisabeth Grüner Schöller
Life of this patent
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Abstract
A method for producing a cheese with reduced amount of galactose comprising inoculating milk with Streptococcus thermophilus Gal(+) bacteria and Lactobacillus Gal(+) Lac(−) bacteria.
Claims14
A method for producing a cheese with a reduced galactose content, comprising:
- (a) inoculating milk with:
- (I) Streptococcus thermophilus (ST) bacteria, wherein the ST bacteria are ST Gal(+) bacteria that reduce galactose content in milk by at least 10% as compared to reference ST strain CHCC4323 (deposited at Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ), under accession number DSM 32826), when assessed by inoculating skim cow milk with 1% overnight cultures of the ST bacteria, incubating for 18 hours at 37° C. to obtain fermented milk, and measuring galactose content in the fermented milk;
- and
- (II) Lactobacillus (Lb.) bacteria, wherein the Lb. bacteria comprise one or more Lb. bacteria selected from Lactobacillus delbrueckii subsp. lactis bacteria and Lb. helveticus bacteria and are Lb. Gal(+)Lac(−) bacteria that are able to ferment galactose but not able to ferment lactose, such that, when 1% overnight cultures of the Lb. bacteria are inoculated in De Man, Rogosa and Sharpe (MRS) medium containing 1% galactose as sole carbohydrate and incubated for 24 hours at 37° C., the pH of the medium has decreased by at least 0.5, and such that, when 1% overnight cultures of the Lb. bacteria are inoculated in MRS medium containing 1% lactose as sole carbohydrate and incubated for 18 hours at 37° C., the pH of the medium has not decreased by more than 1.0;
- (b) fermenting the milk with the bacteria of (a);
- and
- (c) processing the fermented milk to obtain cheese, wherein the cheese has a reduced galactose content.
4 more elements
Dependencies are inferred from the claim text.