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AssigneeJiangnan University
InventorsZhemin Zhou, Zhongmei Liu, Yufeng Liu, Li Zhou, Wenjing Cui, Junling Guo
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Disclosed is a double enzyme tandem preparation method of L-2-aminobutyric acid, and belongs to the field of bioengineering. In the disclosure, recombinant Escherichia coli expressing L-glutamate mutase and recombinant Escherichia coli expressing L-aspartate-β-decarboxylase are separately cultured to obtain L-glutamate mutase and L-aspartate-β-decarboxylase. The two enzymes are added to a reaction system at a certain mass ratio, and L-glutamate is used as a substrate to carry out an enzyme reaction to prepare the L-2-aminobutyric acid. When the dosage of the L-aspartate-β-decarboxylase is 2 mg/mL, and the reaction time is 24 h, 8.5 mmol/L L-2-aminobutyric acid is produced by conversion, with a molar conversion rate of 85.00%. Compared with a chemical production method, the method disclosed by the disclosure has a safe production process and no environmental pollution. Compared with a multi-enzyme synthesis system with threonine as a substrate, the substrate is cheaper and the process is simpler.
A method for biosynthesizing L-2-aminobutyric acid, comprising using L-glutamate as a substrate and a double enzyme tandem reaction system to catalyze L-glutamate to produce L-2-aminobutyric acid, wherein the two enzymes in the double enzyme tandem reaction system are L-glutamate mutase and L-aspartate-B-decarboxylase, wherein the amino acid sequence of the L-aspartate-B-decarboxylase is set forth in SEQ ID NO:1 or SEQ ID NO:2 or SEQ ID NO:3 or SEQ ID NO:4;
Dependencies are inferred from the claim text.