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US 12,031,163 B2Granted
Liquid formulation of alpha-amylase
AssigneeBASF Enzymes LLC
InventorYun Han
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to liquid enzyme formulations containing one or more alpha-amylases for use in starch processing, wherein the pH of the enzyme formulation is about pH 6.0-8.0, and methods of use thereof. The present disclosure further relates to methods of making a liquid enzyme formulation containing one or more alpha-amylase having improved stability, comprising titrating the pH of the liquid enzyme formulation to a range of pH 6.0-8.0.
Claims19
A liquid alpha-amylase enzyme formulation comprising:
- (a) an alpha-amylase having at least 90% sequence identity to an amino acid sequence as set forth in SEQ ID NO:
- 1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, SEQ ID NO:
- 13 or SEQ ID NO:
- 14;
- (b) a buffering agent;
- (c) a stabilizer present at 30% to 50% w/v;
- and
- (d) a preservative, wherein the preservative is potassium sorbate, sodium sorbate, sorbic acid, sodium benzoate, benzoic acid, methyl paraben, calcium propionate, sodium propionate, ammonium propionate, propionic acid, or any combination thereof,
- wherein the pH of the enzyme formulation is 6.3-6.7, and
- wherein the alpha-amylase retains at least 90% of its activity at a temperature of 4-40° C. for at least one year.
10 more elements
Method of using a liquid alpha-amylase enzyme formulation, comprising:
- (a) providing a liquid enzyme formulation comprising:
- (i) an alpha-amylase having at least 90% sequence identity to an amino acid sequence as set forth in SEQ ID NO:
- 1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, SEQ ID NO:
- 13 or SEQ ID NO:
- 14,
- (ii) a buffering agent,
- (iii) a stabilizer present at 30% to 50% w/v, and
- (iv) a preservative,
- wherein the pH value of the liquid enzyme formulation is 6.3-6.7,
- wherein the preservative is potassium sorbate, sodium sorbate, sorbic acid, sodium benzoate, benzoic acid, methyl paraben, calcium propionate, sodium propionate, ammonium propionate, propionic acid, or any combination thereof;
- (b) providing a starch;
- and
- (c) contacting the liquid enzymatic formulation with the starch, thereby hydrolyzing the starch.
13 more elements
Dependencies are inferred from the claim text.