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US 12,031,127 B2Granted
Multiplexed analysis of neuron projections by sequencing
AssigneeCold Spring Harbor Laboratory
InventorsAnthony Zador, Justus Kebschull
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Abstract
The present invention provides a composition comprising a plurality of labeled neurons, each of which is labeled by an expression construct that encodes a unique barcoded nucleic acid.
Claims17
A composition comprising a plurality of labeled neurons, each of which contains
- (a) a barcoded nucleic acid which serves as a label;
- and
- (b) a chimeric protein containing
- (i) a nucleic acid binding domain that is specifically bound to the barcoded nucleic acid in the labeled neuron;
- and
- (ii) a synaptic trafficking signal sequence capable of directing transport of the barcoded nucleic acid to the axon terminals of the labeled neuron, wherein at least 50% of the plurality of labeled neurons are labeled with a unique barcoded nucleic acid, and wherein the barcoded nucleic acid contains a binding region that is specifically recognized by the nucleic acid-binding domain of the chimeric protein.
3 more elements
Dependencies are inferred from the claim text.