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US 12,031,122 B2Granted
Recombinant bacterial cells and methods for producing poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
AssigneeGenecis Bioindustries Inc.
InventorAdam William Westbrook
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Abstract
The disclosure provides recombinant bacterial host cells that metabolize and convert glycerol or volatile fatty acids (VFAs) to poly(3-hydroxybutyrate-co-3-hydroxyvalerate) or PHBV. The disclosure further provides methods of producing PHBV using the recombinant bacteria disclosed herein.
Claims29
A bacterial host cell, comprising the following nucleic acid molecules integrated into the bacterial host cell genome:
- (a) a first operon, comprising:
- (i) a nucleic acid molecule encoding a PhaC protein, wherein the PhaC protein is a Cupriavidus sp. S-6 PhaC protein,
- (ii) a nucleic acid molecule encoding a PhaA protein, wherein the PhaA protein is a Cupriavidus sp. S-6 PhaA protein,
- (iii) a nucleic acid molecule encoding a PhaB protein, wherein the PhaB protein is a Cupriavidus sp. S-6 PhaB protein,
- wherein the first operon comprises a first promoter;
- and
- (b) a second operon, comprising:
- (iv) a nucleic acid molecule encoding a BktB protein, wherein the BktB protein is a Cupriavidus sp. QJ1 BktB protein and
- (v) a nucleic acid molecule encoding a PhaB protein, wherein the PhaB protein is a Cupriavidus sp. S-6 PhaB protein
- wherein the second operon comprises a second promoter,
- wherein the bacterial host cell comprises an activated sleeping beauty mutase (Sbm) pathway;
- and
- wherein the first promoter and the second promoter are the same, and wherein each of the first promoter and the second promoter comprises the nucleic acid sequence of SEQ ID NO:
- 233.
11 more elements
A bacterial host cell, comprising:
- a first operon comprising (a) a nucleic acid molecule encoding a PhaC protein, wherein the nucleic acid molecule comprises a sequence having at least 90% identity to SEQ ID NO:
- 250, (b) a nucleic acid molecule encoding a PhaA protein, wherein the nucleic acid molecule comprises a sequence having at least 90% identity to SEQ ID NO:
- 248, (c) a nucleic acid molecule encoding a PhaB protein, wherein the nucleic acid molecule comprises a sequence having at least 90% identity to SEQ ID NO:
- 249;
- a second operon comprising:
- (i) a nucleic acid molecule encoding a BktB protein, wherein the nucleic acid molecule comprises a sequence having at least 90% identity to SEQ ID NO:
- 251, and (ii) a nucleic acid molecule encoding a PhaB protein, wherein the nucleic acid molecule comprises a sequence having at least 90% identity to SEQ ID NO:
- 249;
- and
- a sleeping beauty mutase (Sbm) operon comprises a Ptrc promoter,
- wherein each of the first and the second operons comprises a promoter comprising the nucleic acid sequence of SEQ ID NO:
- 233.
9 more elements
Dependencies are inferred from the claim text.