Compositions and methods for obtaining organoids
AssigneeStemcell Technologies Canada Inc.
InventorsCharis-Patricia Segeritz, Ryan Conder, Michael Riedel
Life of this patent
Estimating the term…
Abstract
A method for obtaining epithelial organoids is provided. In one embodiment, the method comprises culturing one or more epithelial ducts, epithelial duct fragments and/or epithelial stem cells isolated therefrom in contact with an extracellular matrix in the presence of a basal medium, wherein the medium is free of FGF and/or nicotinamide. Organoids obtained by the methods described herein, and uses thereof, are also provided.
Claims8
A medium for obtaining an epithelial organoid, the medium comprising a basal medium and a Wnt agonist, wherein the medium is (a) free from FGF and/or nicotinamide and (b) free from an inhibitor of BMP signalling and wherein the medium is capable of supporting the organoids for 5 or more passages.
A medium for obtaining an epithelial organoid, the medium comprising a basal medium and one or more of a B27 component, a N2 component, and/or N-Acetylcysteine, wherein the medium is:
- i) free of FGF and/or nicotinamide, free of an activator of the Wnt-beta-catenin pathway, free of a member of the EGF family and free of an inhibitor of BMP signaling, or
- ii) free of FGF and/or nicotinamide, free of a member of the EGF family, free of an inhibitor of BMP signaling, and supplemented with one or more activators of the Wnt-beta-catenin pathway, or
- iii) free of FGF and/or nicotinamide, free of an inhibitor of BMP signaling, and supplemented with one or more activators of the Wnt-beta-catenin pathway and one or more members of the EGF family, and
- wherein the medium supports the organoids for 5 or more passages.
Dependencies are inferred from the claim text.