US 12,029,204 B2 · Transgenic animals and transgenic embryos producing an engineered nuclease

Abstract

The present specification relates to a transgenic animal and a transgenic embryo producing components of an engineered nuclease. According to the disclosure of the present specification, the transgenic animal (or embryo) producing components of an engineered nuclease is a transgenic animal (or embryo) which includes a first cell having a genome including a first toolbox; and a second cell having a genome including a second toolbox, wherein the first toolbox and the second toolbox include at least one of a polynucleotide encoding an RNA-guided endonuclease and a polynucleotide encoding a guide nucleic acid that is able to specifically bind to a target site, respectively, wherein the first toolbox is present in a first locus of the genome of the first cell; the second toolbox is present in a second locus of the genome of the second cell; and the first locus is different from the second locus.

Claims9

Dependent claims

A transgenic bovine animal comprising a plurality of cells having a genome comprising a polynucleotide comprising:

a first Inverted Terminal Repeat (ITR) sequence, a sequence encoding a Cas9 protein, and a second ITR sequence,
wherein the sequence encoding the Cas9 protein is located between the first ITR sequence in a 5′ direction and the second ITR sequence in a 3′ direction,
wherein the polynucleotide is located on at least one locus selected from:
a locus located between KCNAB 1 gene and GMPS gene;
a locus located between CHAFIB gene and PIGP gene;
a locus located between HNRNPR gene and LUZP1 gene;
a locus located between MRPL22 gene and HAVCR1 gene;
a locus located between STMN4 gene and CHRNA2 gene;
a locus located between TGS1 gene and LYN gene;
a locus located between H3F3C gene and TFB2M gene;
a locus located between CLIP3 gene and OVOL3 gene;
and
a locus located between MGC134232 gene and PHKA2 gene,
wherein the plurality of cells express the Cas9 protein such that the transgenic bovine animal expresses the Cas9 protein.

11 more elements

Dependencies are inferred from the claim text.

Transgenic animals and transgenic embryos producing an engineered nuclease

Life of this patent

Transgenic animals and transgenic embryos producing an engineered nuclease

Life of this patent

Abstract

Claims9