Methods and systems for detecting prostaglandins by LC-MS/MS
AssigneeLaboratory Corporation of America Holdings
InventorsBrett Holmquist, Mary Katherine Morr Kelemen
Life of this patent
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Abstract
Disclosed are methods, systems, and computer program products for using liquid chromatography/tandem mass spectrometry (LC-MS/MS) for the analysis of endogenous biomarkers, such as PGD2, in a biological sample. More specifically, the methods, systems, and computer program products are described for detecting and quantifying the amount of an PGD2 in a sample. The quantitative analysis may be helpful in making clinical diagnoses.
Claims21
A method for determining the presence or amount of PGD2 in a biological sample by tandem mass spectrometry, comprising:
- (a) obtaining a sample from a subject;
- (b) adding a stable isotope labeled PGD2 to the sample as an internal standard;
- (c) performing liquid chromatography to purify the sample;
- and
- (d) measuring the PGD2 by tandem mass spectrometry.
A system for determining the presence or amount of at least one biomarker of interest in a biological sample, the system comprising:
- station for providing a test sample suspected of containing PGD2;
- a station for partially purifying PGD2 from other components in the sample;
- a station for chromatographically separating PGD2 from other components in the sample;
- and
- a station for analyzing the chromatographically separated PGD2 by mass spectrometry to determine the presence or amount of PGD2 in the biological sample.
A computer-program product tangibly embodied in a non-transitory machine-readable storage medium, including instructions configured to cause one or more computers to perform actions to measure the presence or amount of PGD2 in a biological sample comprising at least one of the following steps:
- (a) obtaining a biological sample from a subject;
- (b) adding a stable isotope-labeled PGD2 to the sample as an internal standard;
- (c) performing liquid chromatography;
- and
- (d) measuring PGD2 by tandem mass spectrometry.
Dependencies are inferred from the claim text.