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US 12,025,611 B2Granted
Methods of performing animal research
AssigneeRecursion Pharmaceuticals, Inc.
InventorJonathan Betts-LaCroix
Life of this patent
Estimating the term…
Abstract
Disclosed herein are devices, systems and methods for performing cell culture using animal-chip hybrids. A cell culture device may comprise a fluid channel portion having a first port at a first end and a second port at a second end, and a first compartment for culturing cells. A continuous or intermittent perfusion of blood from an animal subject may enter the cell culture device at the first port and exit the cell culture device at the second port.
Claims20
A method for processing or analyzing biological material, the method comprising:
- providing a hybrid in vitro-in vivo cell culture device comprising a housing that includes a drug perfusion compartment separated from a cell culture compartment by a semi-permeable layer, a plenum separated from the cell culture compartment by an additional semi-permeable layer, the plenum comprising a channel having an inlet and an outlet;
- attaching the housing to a body of a subject to perfuse the channel of the hybrid in vitro-in vivo cell culture device with bodily fluids of the subject through the inlet of the channel, such that the channel is in fluid communication with a circulatory system of the subject and the bodily fluids of the subject flows through the inlet of the channel to the plenum first;
- attaching a drug reservoir to the hybrid in vitro-in vivo cell culture device by:
- connecting an inlet channel of the drug reservoir to a first device port such that the first device port is in fluid communication with a drug of the drug reservoir and the first device port introduces a portion of the drug into the drug perfusion compartment;
- and
- connecting an outlet channel of the drug reservoir to a second device port such that the second device port is in fluid communication with the drug reservoir and the second device port removes the portion of the drug from the hybrid in vitro-in vivo cell culture device;
- and
- perfusing the drug perfusion compartment with the portion of the drug from the drug reservoir, the drug perfusion compartment separated from the cell culture compartment by the semi-permeable layer, such that a portion of the drug from the drug perfusion compartment passes through the semi-permeable layer to interact with one or more cells of the bodily fluids in the cell culture compartment.
5 more elements
A device for processing or analyzing biological material, the device comprising:
- a hybrid in vitro-in vivo cell culture device comprising a housing that includes a drug perfusion compartment separated from a cell culture compartment by a semi-permeable layer, a plenum separated from the cell culture compartment by an additional semi-permeable layer, the plenum comprising a channel having an inlet and an outlet;
- wherein the housing, when attached to a body of a subject, is operable to perfuse the channel of the hybrid in vitro-in vivo cell culture device with bodily fluids of the subject through the inlet of the channel, such that the channel is in fluid communication with a circulatory system of the subject and the bodily fluids of the subject flows through the inlet of the channel to the plenum first;
- wherein a drug reservoir when attached to the hybrid in vitro-in vivo cell culture device by an inlet channel of the drug reservoir connected to a first device port in fluid communication with a drug of the drug reservoir is operable to introduce a portion of the drug into the drug perfusion compartment;
- wherein the drug reservoir when attached to the hybrid in vitro-in vivo cell culture device by an outlet channel of the drug reservoir connected to a second device port in fluid communication with the drug reservoir is operable to remove the portion of the drug from the hybrid in vitro-in vivo cell culture device;
- and
- wherein the drug perfusion compartment is separated from the cell culture compartment by the semi-permeable layer, such that when the drug perfusion compartment is perfused with the drug from the drug reservoir, the portion of the drug from the drug perfusion compartment passes through the semi-permeable layer to interact with one or more cells of the bodily fluids in the cell culture compartment.
3 more elements
Dependencies are inferred from the claim text.