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US 12,025,550 B2Granted
Fluidic cartridge for cytometry and additional analysis
AssigneeCytoChip Inc.
InventorsWendian Shi, Yuzhe Ding
Life of this patent
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Abstract
The disclosure relates to devices and methods for analyzing particles in a sample. In various embodiments, the present disclosure provides devices and methods for cytometry and additional analysis. In various embodiments, the present disclosure provides a cartridge device and a reader instrument device, wherein the reader instrument device receives, operates, and/or actuates the cartridge device. In various embodiments, the present disclosure provides a method of using a device as disclosed herein for analyzing particles in a sample.
Claims8
A method for analyzing a sample, comprising:
- applying the sample to a cartridge device, comprising:
- an inlet configured for receiving the sample into the cartridge device;
- a fluidic structure fluidly connected to the inlet and configured for mixing at least a portion of the sample with at least a portion of a reagent to form one or more sample mixtures;
- a flow cell fluidly connected to the fluidic structure, wherein the flow cell is configured for forming one or more sample streams from the one or more sample mixtures and for measuring a signal from the one or more sample streams to detect target particles;
- and
- a flow sensor fluidly connected to the flow cell and configured for measuring a sensing signal from the sample streams that enter the flow sensor;
- mixing at least a portion of the sample and at least a portion of the reagent to form one or more sample mixtures;
- forming one or more sample streams from the one or more sample mixtures in the flow cell;
- and
- measuring the signal from the one or more sample streams in the flow cell to detect the target particles;
- wherein:
- the reagent comprises a fluorescent labeling agent that selectively labels the target particles in the sample with fluorescence;
- and
- a fluidic connection between the flow cell and the flow sensor is configured for forming a sample stream to have the same flow rate flowing through the flow cell and the flow sensor.
11 more elements
Dependencies are inferred from the claim text.