Method for preparing nitrogen-containing heterocyclic compound and derivative thereof by enzymatic-chemical cascade method

A method for preparing a nitrogen-containing heterocyclic compound and a derivative thereof by an enzymatic-chemical cascade method, comprising:

• reacting an alcohol, an amine, an alcohol dehydrogenase, a flavin molecule and a coenzyme in a solvent to obtain the nitrogen-containing heterocyclic compound and the derivative thereof;
• wherein, the nitrogen-containing heterocyclic compound is selected from the group consisting of
• the alcohol is selected from the group consisting of benzyl alcohol, p-methoxybenzyl alcohol, 2-furanmethanol, 2-thiophene methanol, 2-pyridine methanol, n-octanol, benzyl alcohol, p-nitrobenzyl alcohol, cyclohexanol, phenylpropanol, 2-amino-1-propanol, cyclohexyl methanol, cinnamyl alcohol and phenylethanol;
• the amine is selected from the group consisting of o-phenylenediamine, o-aminophenol, 2-amino-1-butanol, 2-amino-1-propanol, 3-aminopropanol, 3-amino-2-methylpropane-1-ol and 6-(3,4 diaminophenyl)-4,5 dihydro-5-methyl-3(2H)-phthalazinone;
• the coenzyme is any one or a combination of NADP+ and NAD+;
• the flavin molecule is any one of the synthetic flavin analog shown in formula I,
• wherein, R1 and R2 are each indendently selected from hydrogen, methyl, trifluoromethyl, methoxy, halogen atom, nitro or amino;
• R3 is selected from hydrogen, C1-C5 alkyl, phenyl or benzyl;
• and X- is selected from halide ion, nitrate or trifluoromethanesulfonate.