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US 12,024,729 B2Granted
Polypeptide having cephalosporin C acylase activity and use thereof
AssigneeAmicogen, Inc.
InventorsZhe Piao, Eun Seon Wang, Hong Xian Li, Mi Kyoung Lee, Ah Reum Son, Su Jin Lim
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Abstract
Disclosed herein are a polypeptide having cephalosporin C(CPC) acylase activity and a use thereof. More specifically, a mutant CPC acylase comprising a point mutation introduced thereinto, thereby exhibiting improved enzymatic activity and/or stability, and a use of the mutant CPC acylase for producing 7-aminocephalosporanic acid (7-ACA), are provided.
Claims18
A mutant cephalosporin C (CPC) acylase, derived from a CPC acylase comprising an alpha-subunit and a beta-subunit,
- wherein the mutant CPC acylase comprises a mutation by substitution of isoleucine at position 179 in the beta-subunit of SEQ ID NO:
- 4 (1179B) with tyrosine (Y) (I179βY), and
- the mutant CPC acylase has an amino acid sequence having 90% or higher identity with SEQ ID NO:1 and retains a CPC acylase function.
Dependencies are inferred from the claim text.