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US 12,024,720 B2Granted
Cell cultivation method, cell support composite production method, cultivated cells, and cell support composite
AssigneeNikkiso Co., Ltd.
InventorFumihiko Kitagawa
Life of this patent
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Abstract
A cell cultivation method includes cultivating dedifferentiated kidney cells in a state of being non-adherent to a culture vessel for a period of 5 days or longer, forming aggregates of the kidney cells during the cultivation period, then cultivating the kidney cells in a state of having formed aggregates, during a portion of the period, and thereby restoring the physiological functions of the kidney cells.
Claims12
A cell cultivation method, comprising:
- cultivating primary cultured kidney cells which are dedifferentiated in a state of being non-adherent to a culture vessel for a period of 5 days or longer,
- forming aggregates of the kidney cells during the cultivation period, then cultivating the kidney cells in a state of having formed aggregates during a portion of the period, and thereby restoring physiological functions of the kidney cells,
- wherein the primary cultured kidney cells are primary cultured renal proximal tubular epithelial cells, wherein a percentage of the primary cultured renal proximal tubular epithelial cells in the primary cultured kidney cells is 83% or more.
A cell cultivation method, comprising:
- cultivating primary cultured kidney cells which are dedifferentiated in a state of being non-adherent to a culture vessel;
- and
- forming aggregates of the kidney cells during the cultivation period, then cultivating the kidney cells in a state of having formed aggregates during a portion of the period, and thereby restoring the physiological functions of the kidney cells,
- wherein the primary cultured kidney cells are primary cultured renal proximal tubular epithelial cells, wherein a percentage of the primary cultured renal proximal tubular epithelial cells in the primary cultured kidney cells is 83% or more.
1 more element
Dependencies are inferred from the claim text.