Synthetic controls for immunohistochemistry
AssigneeGenentech, Inc.
InventorsKathy Hötzel, Franklin V. Peale, Jr., Charles Havnar, Linda Rangell, Debra Y. Dunlap
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Abstract
Provided herein are methods for generating a solid antigen/carrier protein gel for immunohistochemistry (IHC) staining, as well as gels, kits and methods of use thereto. In particular, the methods, gels and kits provided herein include a purified antigen such as a polypeptide antigen, and a carrier protein such as a serum albumin protein, an egg white protein or mixture of egg white proteins, gelatin, or poly-lysine. Examples are provided in which the purified antigen is cross-linked to the carrier protein in the solid antigen/carrier protein gel.
Claims10
A method for generating a solid antigen/carrier protein gel for immunohistochemical (IHC) staining, the method comprising:
- (a) mixing a purified antigen with a liquid solution comprising a carrier protein to produce an antigen/carrier protein liquid solution;
- (b) cross-linking the antigen with the carrier protein using a cysteine-reactive reagent;
- and
- (c) heating the antigen/carrier protein liquid solution to form the solid antigen/carrier protein gel,
- wherein the antigen is a polypeptide antigen comprising a C-terminal cysteine,
- wherein the carrier protein consists of a serum albumin protein, and
- wherein the antigen/carrier protein liquid solution further comprises formaldehyde.
Dependencies are inferred from the claim text.