Peptide nucleic acid functionalized hydrogel microneedles for sampling and detection of interstitial fluid nucleic acids
AssigneesMassachusetts Institute of Technology, Imperial College Innovations Limited
InventorsDarrell J. Irvine, Jason Y. H. Chang, Sylvain Ladame, Dana Al Sulaiman
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Abstract
The present disclosure relates to a device, comprising a base and a plurality of microneedles attached to the base, wherein each microneedle has an outer surface; the outer surface of at least one microneedle being coated with a composition comprising at least one polymer and least one Peptide Nucleic Acid (PNA). The present disclosure additionally relates to a method of detecting an analyte in interstitial fluid (ISF), comprising contacting the device to a subject, for example, to human skin.
Claims24
A device for detecting an analyte, comprising a base, and a plurality of microneedles attached to the base, wherein:
- each microneedle has an outer surface;
- and
- the outer surface of at least one microneedle is coated with a composition comprising at least one polymer and at least one first Peptide Nucleic Acid (PNA),
- wherein the polymer is covalently attached to the first PNA, by a first linker, wherein the first linker is a photocleavable linker represented by structural formula (II) or (IIa),
- wherein indicates a point of attachment of the first linker to the polymer or to the first PNA.
Dependencies are inferred from the claim text.