US 12,018,311 B2 · Long chain dibasic acid with low content of long chain dibasic acid impurity of shorter carbon-chain and preparation method thereof

Abstract

The present invention relates to a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain, to the preparation of a long-chain dibasic acid producing strain by directed evolution of POX gene and homologous recombination, and to the production of a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain by using the strain. The present invention also relates to a strain containing a mutated promoter, wherein, when a long-chain dibasic acid is produced by fermentation of this strain, the content of the acid impurity of shorter carbon chain in the fermentation product is significantly reduced.

Claims15

Dependent claims

A method of producing a long chain dibasic acid or a fermentation broth by fermentation using a microorganism, comprising culturing the microorganism, and optionally isolating and/or purifying the long chain dibasic acid from the culture product,

wherein the microorganism comprising an isolated mutated POX gene or a variant thereof,
wherein said isolated mutated POX gene or a variant thereof comprises:
[i] a nucleic acid sequence having at least 80% sequence identity to SEQ ID NO:24, and [ii] a deletion of any one nucleotide base in the promoter of said POX gene from nucleic acid residues 266-275 of SEQ ID NO:24.

Dependencies are inferred from the claim text.

Facts

Application

17/814,972Filed 26 Jul 2022 · division

Priority

CN 201810734151.86 Jul 2018

Published

US 2022/0380816 A11 Dec 2022

Granted

25 Jun 2024

Term ends

Not estimatedThe source records don’t support an estimate.

Classification11 codes

C12P 7/44
Containing a carboxyl group including Peroxycarboxylic acids: polycarboxylic acids
C12P 7/6409
Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group;…:…
C12N 9/001
Oxidoreductases: acting on the CH-CH group of donors
C12N 15/01
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or…:…

Long chain dibasic acid with low content of long chain dibasic acid impurity of shorter carbon-chain and preparation method thereof

Life of this patent

Long chain dibasic acid with low content of long chain dibasic acid impurity of shorter carbon-chain and preparation method thereof

Life of this patent

Abstract

Claims15