Optimization of C-5 sterol desaturation
AssigneeDsm IP Assets B.V.
InventorsChristopher Mark Farrell, Lisa Ann Laprade, Otto Martin Lehmann, Joshua Trueheart, Bastien Jean Wolfgang Chevreux
Life of this patent
Estimating the term…
Abstract
The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified host strains expressing enzymes having improved C-5 sterol 5 desaturase activity and their use in a process for production of vitamin D3 or derivatives and/or metabolites thereof.
Claims13
A cholesterol-producing yeast cell expressing (i) a heterologous C5-sterol desaturase with at least 90% sequence identity to SEQ ID NO:
- 2, 4, 6 or 8 and (ii) a heterologous C8-isomerase with at least 90% sequence identity to SEQ ID NO:_14, wherein the yeast cell is capable of producing a sterol mix comprising 7-dehydrocholesterol (7-DHC), lanosterol, and lathosterol, wherein the ratio of 7-DHC to lanosterol and lathosterol combined is at least 5% higher than the ratio of 7-DHC to lanosterol and lathosterol combined in a sterol mix produced by a reference yeast cell that does not express the heterologous C5-sterol desaturase and C8-isomerase.
Dependencies are inferred from the claim text.