Process for producing a glucuronide and genetically modified microorganisms useful in this process
AssigneeUtah State University
InventorJixun Zhan
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Abstract
The present invention relates to an in vitro or in vivo process for producing a glucuronide comprising a glucuronic acid moiety bound to a phenolic hydroxyl group or a phenolic carboxyl group. Also provided are expression vectors, nucleic acids, polypeptides, and recombinant microbial cells useful in carrying out the process and prodrugs produced by the process.
Claims11
A process for producing a glucuronide from UDP-glucuronic acid and a phenolic compound that comprises a hydroxyl or a carboxyl group, wherein said glucuronide comprises a glucuronic acid moiety bound to the hydroxyl or the carboxyl group of said phenolic compound, wherein said process comprises the steps of:
- (i) culturing a genetically modified microorganism in a medium that comprises the phenolic compound, wherein the genetically modified microorganism expresses a UDP-glucuronyltransferase (UGT) that comprises the amino acid sequence of SEQ ID NO:7 or an amino acid sequence at least 95% identical to SEQ ID NO:7, under conditions allowing the genetically modified microorganism to produce the glucuronide;
- and
- (ii) optionally recovering the glucuronide from the medium.
Dependencies are inferred from the claim text.