Process for production of nigericin from Streptomyces sp. MCC-0151

A process for synthesis of nigericin from Streptomyces sp. MCC 0151, the process comprising:

• (i) providing a pure colony of Streptomyces sp. MCC 0151;
• (ii) preparing an inoculum by incubating the pure colony of Streptomyces sp. MCC 0151 in liquid cultivation medium (LCM);
• (iii) providing a metabolite production medium;
• (iv) aseptically inoculating the inoculum from (ii) to the metabolite production medium and culturing the inoculum therein;
• (v) mixing the metabolite production medium containing the Streptomyces sp. MCC 0151 and ethyl acetate in a ratio of 1:1 to obtain a mixture;
• (vi) centrifuging the mixture obtained in (v) to obtain a supernatant and an ethyl acetate extract comprising nigericin;
• (vii) evaporating the ethyl acetate extract obtained in (vi) to obtain a dried crude extract;
• and
• (viii) purifying the dried crude extract obtained in (vii) by column chromatography to obtain a purified nigericin, wherein nigericin is produced having a high yield with concentration up to 500 mg/L.