Genetically optimised microorganism for producing molecules of interest
AssigneeEppen Europe
InventorsCedric Boisart, Nicolas Morin
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Abstract
The invention concerns a genetically modified microorganism expressing a functional type I or II RuBisCO enzyme and a functional phosphoribulokinase (PRK), and in which the glycolysis pathway is at least partially inhibited, said microorganism being genetically modified so as to produce an exogenous molecule and/or to overproduce an endogenous molecule. According to the invention, the oxidative branch of the pentose phosphate pathway may also be at least partially inhibited. The invention also concerns the use of such a genetically modified microorganism for the production or overproduction of a molecule of interest and processes for the synthesis or bioconversion of molecules of interest.
Claims11
Genetically modified microorganism expressing a functional RuBisCO enzyme and a functional phosphoribulokinase (PRK), and in which glycolysis pathway is inhibited by inhibiting the expression of a gene encoding glyceraldehyde 3-phosphate dehydrogenase and/or a gene encoding phosphoglycerate kinase, and oxidative branch of pentose phosphate pathway of said microorganism is also inhibited by inhibiting the expression of a gene encoding glucose-6-phosphate dehydrogenase or 6-phosphogluconolactonase or 6-phosphogluconate dehydrogenase, said microorganism being genetically modified so as to produce an exogenous molecule of interest and/or to overproduce an endogenous molecule of interest, other than the RuBisCO or phosphoribulokinase enzyme.
Dependencies are inferred from the claim text.