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US 12,018,286 B2Granted
Generating human podocyte cells
AssigneeThe Cleveland Clinic Foundation
InventorsOliver Wessely, Uyen Wessely, Jan Jensen, Michael Bukys
Life of this patent
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Abstract
Provided herein are compositions, systems, kits, and methods for generating human podocyte cells by contacting human nephron progenitor cells with an FGFR pathway inhibitor, a BMP pathway inhibitor, and a WNT pathway inhibitor. In certain embodiments, the nephron progenitor cells are further contacted with at least one factor selected from: BMP4, BMP7, lysophosphatidic acid, and gamma-secretase inhibitor XX. In certain embodiments, the contacting the nephron progenitor cells is performed under serum-free conditions.
Claims12
A method of generating podocyte cells comprising:
- a) contacting a population of nephron progenitor cells with an FGFR pathway inhibitor, a BMP pathway inhibitor, a γ secretase inhibitor BMP4, BMP7, and a WNT pathway inhibitor;
- and
- b) culturing at least a portion of said population of nephron progenitor cells such that a population of podocyte cells is generated.
Dependencies are inferred from the claim text.