CD62L specific lentiviral vector particle for targeted transduction of T cell subsets

A composition comprising:

• i) a pseudotyped retroviral vector particle that expresses:
• a) a first envelope protein of a Paramyxoviridae family virus that has antigen binding activity, wherein the first envelope protein is selected from protein G, protein HN and protein H;
• wherein the first envelope protein has been modified whereby it does not bind to naturally occurring receptors for said virus on host cells,
• wherein said first envelope protein is fused at its ectodomain to an antigen binding domain that specifically binds a tag of a tagged polypeptide;
• b) a second envelope protein of said Paramyxoviridae family virus that has fusion activity;
• ii) T cells expressing cell-surface CD62L, and
• iii) said tagged polypeptide, wherein said tagged polypeptide binds specifically to CD62L.

A method for transducing T cells that express cell surface CD62L, the method comprising contacting said T cells in a medium with a pseudotyped retroviral vector particle,

• wherein the pseudotyped retroviral vector particle expresses:
• a) a first envelope protein of a Paramyxoviridae family virus that has antigen binding activity, wherein the first envelope protein is selected from protein G, protein HN and protein H;
• wherein the first envelope protein has been modified whereby it does not bind to naturally occurring receptors for said virus on host cells,
• wherein said first envelope protein is fused at its ectodomain to an antigen binding domain that specifically binds CD62L;
• and
• b) a second envelope protein of said Paramyxoviridae family virus that has fusion activity;
• whereupon said T cells are transduced by the pseudotyped retroviral vector particle, irrespective of whether shed CD62L (sCD62L) is present in the medium.

A method for transducing T cells that express cell-surface CD62L, the method comprising contacting said T cells in a medium with a pseudotyped retroviral vector particle and with a tagged polypeptide that specifically binds CD62L,

• wherein the pseudotyped retroviral vector particle expresses:
• a) a first envelope protein of a Paramyxoviridae family virus that has antigen binding activity, wherein the first envelope protein is selected from protein G, protein HN and protein H;
• wherein the first envelope protein has been modified whereby it does not bind to naturally occurring receptors for said virus on host cells,
• wherein said first envelope protein is fused at its ectodomain to an antigen binding domain that specifically binds a tag on said tagged polypeptide;
• and
• b) a second envelope protein of said Paramyxoviridae family virus that has fusion activity;
• whereupon said T cells are transduced by the pseudotyped retroviral vector particle, irrespective of whether shed CD62L (sCD62L) is present in the medium.