Concurrent analysis of multiple analytes
AssigneeThe Regents of the University of California
InventorsAustin Quach, Kym Francis Faull
Life of this patent
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Abstract
Methods, apparatuses and systems are described that are capable of simultaneously determining the presence, identities or levels of multiple analytes present in a single sample, by carrying out steps including denaturation, normalization, extraction, mixed-mode liquid chromatography and mass spectrometry, whereby the presence, identities or levels of analytes in the single sample are determined.
Claims20
A method for substantially simultaneously determining the presence, identities or amounts of a plurality of analytes present in a single sample, the method comprising:
- a. treating the sample with a denaturation treatment thereby forming a denatured sample, wherein the denaturation treatment does not denature components of a normalization reagent when added thereto;
- b. treating the denatured sample with the normalization reagent, capable of being separated by multiple modes of chromatography and individually thereby forming a normalized sample;
- c. treating the normalized sample with an extraction reagent and retaining soluble analyte species and analyte fragment species therein, thereby forming an extracted sample;
- d. subjecting the extracted sample to an instrument to identify the plurality of analytes or derivatives thereof, thereby identifying the plurality of analytes;
- and
- e. computationally determining from the data the presence, identities or amounts of the plurality of analytes present in the extracted sample.
A system for substantially simultaneously determining the presence, identities or amounts of a plurality of analytes present in a single sample, the system comprising:
- (i) a denaturation reagent, wherein the denaturation reagent does not denature components of a normalization reagent when added thereto;
- (ii) a normalization reagent that converts analytes in the sample into normalized analyte species or normalized analyte fragment species;
- (iii)an extraction reagent;
- (iv)a separation apparatus;
- (v) a first instrument configured to generate data on the normalized analyte species and normalized analyte fragment species;
- and
- (vi) a second instrument configured to computationally determine from the data the presence, identities or amounts of the plurality of analytes present in the sample.
An apparatus for substantially simultaneously determining the presence, identities or amounts of a plurality of analytes present in a single sample, the apparatus comprising:
- (i) means for denaturing biopolymers in the sample, wherein the denaturing does not denature components of a normalization reagent when added thereto;
- (ii) means for normalizing the sample;
- (iii) means for extracting the normalized sample to obtain analyte species and analyte fragment species therein;
- (iv) means for identifying and computationally determining the presence, identities or amounts of the plurality of analytes present in the sample.
Dependencies are inferred from the claim text.