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US 12,011,016 B2Granted
Protein methods and compositions
AssigneeImpossible Foods Inc.
InventorsCarl Allen Henderson, Jr., Ratnayake Mudiyanselage Dunilka Nishani Ratnayake, Ian Guiles Ronningen, Laila Dafik
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Abstract
This disclosure describes methods for purifying protein, and more particularly to methods for purifying protein that minimize the development of undesirable odors and flavors in the purified protein and increase protein yield.
Claims27
A method for purifying proteins from a plurality of cells having cell walls, the method comprising:
- a) perforating the cell walls of the plurality of cells;
- b) separating an aqueous suspension of the perforated plurality of cells of a) to form a solids portion and a liquid portion;
- c) filtering the liquid portion to form a filtrate and a retentate;
- and
- d) concentrating the retentate to form a protein composition;
- wherein each of a)-d), independently, is performed at a pH between about 8.5 and about 12.0, inclusive;
- and
- wherein the perforating comprises treating with a reductant, treating with an enzyme, electroporation, or a combination thereof.
5 more elements
Dependencies are inferred from the claim text.