# US 12,010,467 B2 · Method and system for whole slide imaging

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- Number: US 12,010,467 B2
- Document ID: US12010467B2
- Status: Granted
- Kind: B2
- Application number: 17/790,291
- Filed: 2020-12-24
- Published: 2023-04-06
- Granted: 2024-06-11
- Earliest claimed: 2019-12-31
- Assignees: Koninklijke Philips N.V.
- Applicants: Koninklijke Philips N.V.
- Inventors: Prarthana Shrestha; Jelte Peter Vink; Maarten Van Lierop; Gerardus Johannes Wilhelmus Van Den Braak; Mathijs Rem; Jeroen De Kleijn
- Web page: https://patentgrep.com/patent/US12010467B2

## CPC

- H04N 1/6033: {using test pattern analysis (H04N1/6055 takes precedence)}
- H04N 9/67: for matrixing (camera processing pipelines for matrixing of colour signals H04N23/85)
- G02B 21/365: {Control or image processing arrangements for digital or video microscopes (G02B21/361, G02B21/362 take precedence)}
- G06T 7/90: Determination of colour characteristics
- G06T 2207/10024: Color image
- G06T 2207/10056: Microscopic image
- G06T 2207/30024: Cell structures in vitro; Tissue sections in vitro
- H04N: PICTORIAL COMMUNICATION, e.g. TELEVISION
- G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G06T: IMAGE DATA PROCESSING OR GENERATION, IN GENERAL

## Abstract

A method (and system) is provided obtaining an adapted calibration transformation for whole slide imaging, wherein the adapted calibration transformation is for outputting the color of an empty area of the slide in a whole slide image, WSI, as a desired color. The method comprises first receiving a color target WSI in a device-dependent color space, wherein the color target WSI is a WSI of a color target from a scanner represented in the device-dependent color space. The color target contains at least a white patch and three (near) primary color patches. A calibration transformation is obtained for transforming the device-dependent color space to a device-independent color space. A chromatic adaptation transformation is derived using the color of the white patch in the device-dependent color space, the color of the empty (glass) area in the device-independent color space and the desired empty (glass) color in the target color space. An adapted calibration transformation is obtained by applying the chromatic adaptation transformation to the calibration transformation.

## Claims

### Claim 1

A method for obtaining an adapted calibration transformation for whole slide imaging, wherein the adapted calibration transformation is for outputting the color of an empty area of the slide in a whole slide image, WSI, obtained by a scanner as a desired color in a device-independent color space, the method comprising:
receiving a color target WSI in a device-dependent color space, wherein the color target WSI is a WSI of a color target from the scanner represented in the device-dependent color space and wherein the color target contains at least a white patch and three color patches;
obtaining a calibration transformation for transforming the device-dependent color space to the device-independent color space;
determining a chromatic adaptation transformation based on the color of the white patch in the device-dependent color space, the color of the empty area in the device-dependent color space and the desired color in a target color space; and
obtaining an adapted calibration transformation based on applying the chromatic adaptation transformation to the calibration transformation; wherein obtaining a calibration transformation is based on calibrating the scanner by mapping a set of scanner acquired raw RGB values from the color target with a set of reference colorimetric values and obtaining a calibration transformation.

### Claim 2 (depends on 1)

The method as claimed in claim 1, wherein the device-independent color space comprises:
the XYZ color space; or
the Lab color space.

### Claim 3 (depends on 1)

The method as claimed in claim 1, wherein the color target consists of at least one white patch and at least three color patches, wherein a color patch includes
an IT 8.7/1 color target,
a Macbeth color checker,
a Sierra color target,
a Chromacal color target, or
a MGH color target.

### Claim 4 (depends on 1, 2, 3)

The method as claimed in claims 1 to 3, wherein determining a chromatic adaptation transformation comprises:
applying the adapted calibration transformation to the color of the empty area in the device-dependent color space;
applying a further transformation to the color of the empty area to the target color space;
determining a color difference between the empty area in the target color space and the desired color in the target color space; and
modifying a white point in the device-independent color space iteratively to obtain an adapted white point in the device-independent color space such that the adapted calibration transformation results in the minimum color difference.

### Claim 5 (depends on 4)

The method as claimed in claim 4, wherein modifying the scanner white point comprises determining a chromatic adaptation matrix, given the white point in the device-independent color space as a source white point and an adapted scanner white point as a destination white point.

### Claim 6 (depends on 1)

The method as claimed in claim 1, wherein the target color space comprises a linear color space, and comprises:
the sRGB color space;
the SWOP CMYK color space;
the Adobe RGB color space; or
the ProPhoto RGB color space.

### Claim 7 (depends on 1)

The method as claimed in claim 1, wherein determining a chromatic adaptation transformation is based on one or more of:
the XYZ scaling method;
the Bradford method; and
the Von Kries method.

### Claim 8 (depends on 1)

The method as claimed in claim 1, wherein the desired color is neutral white and the target color space is the linear sRGB color space.

### Claim 9 (depends on 1)

A non-transitory computer readable medium comprising code means for implementing the method of claim 1 when said program is run on a processing system.

### Claim 10

A system for obtaining an adapted calibration transformation for whole slide imaging, wherein the adapted calibration transformation is for outputting the color of an empty area of the slide in a whole slide image WSI obtained by a scanner as a desired color in a device-independent color space, the system comprising:
a scanner for obtaining a WSI in a device-dependent color space;
a processor configured to:
receive a color target WSI in a device-dependent color space, wherein the color target WSI is a WSI of a color target from the scanner represented in the device-dependent color space and wherein the color target contains at least a white patch and three color patches;
obtain a calibration transformation for transforming the device-dependent color space to a device-independent color space based on calibrating the scanner by mapping a set of scanner acquired raw RGB values from the color target with a set of reference colorimetric values to obtaining the calibration transformation;
determine a chromatic adaptation transformation based on the color of the white patch in the device-dependent color space, the color of the empty area in the device-dependent color space and the desired color in a target color space; and
obtain an adapted calibration transformation based on applying the chromatic adaptation transformation to the calibration transformation.

### Claim 11 (depends on 10)

The system as claimed in claim 10, wherein the device-independent color space comprises:
the XYZ color space; or
the Lab color space,
and wherein the color target consists of at least one white patch and at least three color patches, and comprises:
an IT 8.7/1 color target;
a Macbeth color checker;
a Sierra color target;
a Chromacal color target; or
a MGH color target.

### Claim 12 (depends on 10)

The system as claimed in claim 10, wherein the processor is adapted to determine a chromatic adaptation transformation by:
applying the adapted calibration transformation to the color of the empty area in the device-dependent color space;
applying a further transformation to the color of the empty area to the target color space;
determining a color difference between the empty area in the target color space and the desired color; and
modifying a white point in the device-independent color space iteratively to obtain an adapted white point in the device-independent color space such that the adapted calibration transformation results in the minimum color difference.

### Claim 13 (depends on 12)

The method as claimed in claim 12, wherein modifying the scanner white point comprises determining a chromatic adaptation matrix, given the white point in the device-independent color space as a source white point and an adapted scanner white point as a destination white point.

### Claim 14 (depends on 11)

The system as claimed in claim 11, wherein the target color space comprises a linear color space, and comprises:
the sRGB color space;
the SWOP CMYK color space;
the Adobe RGB color space; or
the ProPhoto RGB color space.