# US 12,009,195 B2 · Systems and methods for securing fabric, paper, and film samples for analysis by laser ablation > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,009,195 B2 - Document ID: US12009195B2 - Status: Granted - Kind: B2 - Application number: 17/381,949 - Filed: 2021-07-21 - Published: 2022-01-27 - Granted: 2024-06-11 - Earliest claimed: 2020-07-22 - Assignees: Elemental Scientific, Inc. - Applicants: Elemental Scientific, Inc. - Inventors: Daniel R. Wiederin; Ross Coenen; Mark Casper - Web page: https://patentgrep.com/patent/US12009195B2 ## CPC - H01J 49/0409: {Sample holders or containers (containers for retaining a material to be analyzed, B01L3/50, for DNA, C12Q1/6834, for biological materials, G01N33/543)} - H01J 49/0418: {for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates} - G01N 1/04: in the solid state, e.g. by cutting - G01N 2001/045: {Laser ablation; Microwave vaporisation} - H01J 49/0463: {Desorption by laser or particle beam, followed by ionisation as a separate step (sample holder per se H01J49/0418)} - H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Systems and methods are described for securing fabric, paper, and film samples for analysis by laser ablation. A method embodiment includes, but is not limited to, securing a thin, solid sample with a sample holder system, the sample holder system configured to hold the thin, solid sample in a taut configuration between a piston and a sample holder base; transferring the sample holder system to a laser ablation system; and ablating at least a portion of the thin, solid sample in the taut configuration with the laser ablation system to provide an ablated sample. ## Claims ### Claim 1 A method for securing a thin, solid sample for laser ablation, the method comprising: securing a thin, solid sample with a sample holder system, the sample holder system configured to hold the thin, solid sample in a taut configuration between a piston and a sample holder base; introducing a coating to the sample holder system to cover at least a portion of the thin, solid sample in the taut configuration; ablating a blank sample holder system with the laser ablation system to provide a blank sample, the blank sample holder system including the coating and having no portions of the thin, solid sample thereon; transferring the sample holder system to a laser ablation system; ablating at least a portion of the thin, solid sample in the taut configuration with the laser ablation system to provide an ablated sample; analyzing the content of the blank sample via a sample analysis system; generating a signal corresponding to the content of the blank sample; analyzing content of the ablated sample via the sample analysis system; generating a signal corresponding to the content of the ablated sample; and subtracting the signal corresponding to the content of the blank sample from the signal corresponding to the content of the ablated sample. ### Claim 2 (depends on 1) The method of claim 1, wherein the sample holder base includes interior sidewalls defining an aperture into which at least a portion of the piston is configured to fit. ### Claim 3 (depends on 2) The method of claim 2, wherein the piston includes a base and a projection extending from the base, wherein at least a portion of the projection is configured to fit within the aperture of the sample holder base to press the thin, solid sample into the taut configuration. ### Claim 4 (depends on 3) The method of claim 3, wherein the projection includes sidewalls configured to fit within the aperture of the sample holder base, and wherein the sample holder system includes a gap defined by the sidewalls of the projection and the interior sidewalls of the sample holder base. ### Claim 5 (depends on 4) The method of claim 4, wherein sample holder system is configured to introduce a portion of the thin, solid sample into the gap defined by the sidewalls of the projection and the interior sidewalls of the sample holder base to hold the thin, solid sample in the taut configuration. ### Claim 6 (depends on 4) The method of claim 4, wherein the gap is from about 0 mm to about 1 mm. ### Claim 7 (depends on 1) The method of claim 1, wherein the sample holder system is configured to hold a thin, solid sample having a thickness from about 0.05 mm to about 1.5 mm. ### Claim 8 (depends on 1) The method of claim 1, wherein the sample analysis system includes at least one of inductively coupled plasma emission spectroscopy, inductively coupled plasma mass spectrometry, optical spectroscopy, laser-induced breakdown spectroscopy, and infrared spectrometry. ### Claim 9 A method for securing a thin, solid sample for laser ablation, the method comprising: directing a laser from a laser ablation system onto a thin, solid sample in a taut configuration held in a taut configuration by a sample holder system to provide an ablated sample, the sample holder system configured to hold the thin, solid sample in the taut configuration between a piston and a sample holder base; transferring the ablated sample via a carrier gas to a sample analysis system; analyzing the content of the ablated sample with the sample analysis system; ablating a blank sample holder system with the laser ablation system to provide a blank sample, the blank sample holder system having no portions of the thin, solid sample thereon; analyzing the content of the blank sample via the sample analysis system; generating a signal corresponding to the content of the blank sample; and subtracting the signal corresponding to the content of the blank sample from the signal corresponding to the content of the ablated sample. ### Claim 10 (depends on 9) The method of claim 9, wherein the sample analysis system includes at least one of inductively coupled plasma emission spectroscopy, inductively coupled plasma mass spectrometry, optical spectroscopy, laser-induced breakdown spectroscopy, and infrared spectrometry. ### Claim 11 (depends on 9) The method of claim 9, wherein the sample holder base includes interior sidewalls defining an aperture into which at least a portion of the piston is configured to fit. ### Claim 12 (depends on 11) The method of claim 11, wherein the piston includes a base and a projection extending from the base, wherein at least a portion of the projection is configured to fit within the aperture of the sample holder base to press the thin, solid sample into the taut configuration.