# US 12,007,390 B2 · Prefabricated microparticle for performing a detection of an analyte > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,007,390 B2 - Document ID: US12007390B2 - Status: Granted - Kind: B2 - Application number: 17/352,958 - Filed: 2021-06-21 - Published: 2021-10-14 - Granted: 2024-06-11 - Earliest claimed: 2016-12-30 - Assignees: Blink AG - Applicants: Blink AG - Inventors: Katrin Steinmetzer; Stephan Hubold; Thomas Ellinger; Eugen Ermantraut; Torsten Schulz - Web page: https://patentgrep.com/patent/US12007390B2 ## CPC - G01N 33/54393: {Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding} - C12Q 1/682: Signal amplification - C12Q 1/6848: characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction - C12Q 1/6851: Quantitative amplification - G01N 33/54306: {Solid-phase reaction mechanisms} - G01N 33/54313: {the carrier being characterised by its particulate form} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES ## Abstract The present invention relates to a prefabricated microparticle for performing detection, preferably a digital detection and/or quantitation of an analyte. Furthermore, it also relates to a detection and/or quantitation of multiple analytes by prefabricated microparticles. It also relates to a collection of such prefabricated microparticles and to the use of such microparticle(s) and/or of such collection. Furthermore, the present invention also relates to a method of performing a detection and/or quantitation of an analyte in a sample wherein a microparticle or collection of microparticles are used. In one embodiment, in the collection of microparticles, individual microparticles are tailored for the detection of specific analytes and can be distinguished from each other by a specific label indicating the respective analyte for which the individual microparticle is specific. ## Claims ### Claim 1 A method of performing a detection of an analyte in a sample, said method comprising the steps: a) providing prefabricated microparticles, each of which has a surface and includes a void volume for receiving an aqueous solution, wherein each of said prefabricated microparticles is dispersible in a non-aqueous medium and, upon dispersion in a non-aqueous medium, provides for a defined reaction space in such non-aqueous medium, in which defined reaction space a chemical or biochemical reaction indicating the presence of an analyte can be performed, and wherein each of said prefabricated microparticles does not comprise a capture agent that selectively and specifically binds the analyte to be detected; wherein each of said microparticles is a porous microparticle and is capable of taking up liquid and an analyte present in said liquid, from surroundings to which it is exposed; and wherein each of said prefabricated microparticles further comprises a detection agent that is specific for the analyte, and that binds said analyte; b) exposing said prefabricated microparticles to an aqueous sample suspected of containing an analyte to be detected, thus allowing the uptake of liquid and of an analyte present in said liquid, in the porous microparticles, i.e. in the space provided for by the pores of said microparticles; and c) placing the prefabricated microparticles into a non-aqueous phase, and using the void volume of each of said prefabricated microparticles as a defined reaction space in which a chemical or biochemical reaction indicating the presence of an analyte, is performed, by either d1) detecting the detection agent bound to said analyte; or d2) amplifying the analyte, if present, by way of an amplification reaction, and detecting the thus amplified product by means of said detection agent, wherein said analyte is a nucleic acid and said amplification reaction is a nucleic acid amplification, or d3) performing a signal amplification reaction and detecting the thus amplified signal, wherein said reaction space in which said chemical or biochemical reaction indicating the presence of an analyte, is performed, is defined by said void volume of each of said prefabricated microparticles and is not larger than said void volume of each of said prefabricated microparticles. ### Claim 2 (depends on 1) The method according to claim 1, wherein said prefabricated microparticles are provided as prefabricated microparticles which are dried. ### Claim 3 (depends on 2) The method according to claim 2, wherein each of said prefabricated microparticles is reconstituted in an aqueous solution either during step a) or step b), and, upon reconstitution, receives such aqueous solution in its void volume. ### Claim 4 (depends on 1) The method according to claim 1, wherein said prefabricated microparticles are not microparticles that are in-situ generated at the site or in the reaction, at or during which analyte detection is to take place. ### Claim 5 (depends on 1) The method according to claim 1, wherein said detection agent is included in said prefabricated microparticles during a prefabrication process or is included in an aqueous solution resulting from reconstitution of the microparticles either during step a) or step b), and thus becomes part of the prefabricated microparticles upon reconstitution. ### Claim 6 (depends on 1) The method according to claim 1, wherein each of said prefabricated microparticles is made of a gel-forming agent, such gel-forming agent being liquefiable upon the application of heat or light, or upon a change of pH, redox potential, ionic strength, temperature, magnetic field or electromagnetic radiation, or upon exposure to an enzyme or, if the gel-forming agent itself comprises an enzyme, to a substrate of such enzyme. ### Claim 7 (depends on 6) The method according to claim 6, wherein said gel-forming agent forms a matrix defining the surface and the void volume of each of said microparticles. ### Claim 8 (depends on 6) The method according to claim 6, wherein said gel-forming agent is selected from a) synthetic polymers prepared from their corresponding monomers; b) silicone-based polymers; and c) naturally occurring polymers selected from polysaccharides, polypeptides, and polynucleotides. ### Claim 9 (depends on 1) The method according to claim 1, wherein said detection agent is selected from antibodies or antibody fragments, nucleic acids, Spiegelmers, and non-antibody proteins, each of them optionally being labelled with a suitable reporter molecule that produces an optically or otherwise detectable signal indicating the presence of the analyte to be detected. ### Claim 10 (depends on 1) The method according to claim 1, wherein each of said prefabricated microparticles is specifically labelled. ### Claim 11 (depends on 1) The method according to claim 1, which is performed using a collection of prefabricated microparticles. ### Claim 12 (depends on 11) The method according to claim 11, wherein, in said collection of prefabricated microparticles, said prefabricated microparticles are different from each other in that they are specific for different analytes to be detected, wherein each prefabricated microparticle is specifically labelled such that different prefabricated microparticles and their corresponding detected analytes can be distinguished by the specific labels of the prefabricated microparticles. ### Claim 13 (depends on 11) The method according to claim 11, wherein the collection of prefabricated microparticles is suspended in the non-aqueous phase and/or is located on a solid substrate isolating each prefabricated microparticle from other prefabricated microparticles, if present, wherein said solid substrate is a filter, a sieve, a substrate having a pattern of wells, recesses, grooves, channels, trenches, craters, holes, or pillars. ### Claim 14 (depends on 1) The method according to claim 1, wherein said method involves the use of prefabricated microparticles or of a collection of prefabricated microparticles, for performing a digital detection of an analyte or a plurality of analytes in a sample or for enriching and concentrating a plurality of analytes in a plurality of defined volumes, wherein all of said defined volumes in said plurality of defined volumes are equal. ### Claim 15 (depends on 1) The method according to claim 1, wherein, after the step of exposing b), there is one or several washing steps. ### Claim 16 (depends on 1) The method according to claim 1, wherein in step a), said prefabricated microparticles are provided in dried form, and, in step b), said prefabricated microparticles are reconstituted in aqueous solution and then exposed to a sample suspected of containing an analyte to be detected, wherein, optionally after the step of reconstituting, there is one or several washing steps. ### Claim 17 (depends on 1) The method according to claim 1, wherein, in step b) a number of prefabricated microparticles and a number of analyte molecules in the sample are maintained or adjusted, as necessary, such that the binding of a single analyte molecule per prefabricated microparticle follows a Poisson distribution such that, on average, there is no more than one analyte molecule bound per microparticle, thus allowing the detection of a single analyte molecule per prefabricated microparticle. ### Claim 18 (depends on 1) The method according to claim 1, wherein, during step c), the prefabricated microparticles are suspended in the non-aqueous phase and/or are located on a solid substrate isolating each prefabricated microparticle from other prefabricated microparticles, if present, wherein said solid substrate is a filter, a sieve, a substrate having a pattern of wells, recesses, grooves, channels, trenches, craters, holes, or pillars. ### Claim 19 (depends on 6) The method according to claim 6, wherein, during or after step c), the gel-forming agent is liquefied through the application of heat or light, or by a change of pH, redox potential, ionic strength, temperature, magnetic field or electromagnetic radiation, or upon exposure to an enzyme or, if the gel-forming agent itself comprises an enzyme, to a substrate of such enzyme, resulting in an aqueous droplet in a non-aqueous phase. ### Claim 20 (depends on 1) The method according to claim 1, wherein the non-aqueous phase is an oil phase.