# US 12,007,359 B2 · Compensation voltage adjustment for ion mobility separation

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 12,007,359 B2
- Document ID: US12007359B2
- Status: Granted
- Kind: B2
- Application number: 17/303,429
- Filed: 2021-05-28
- Published: 2022-12-01
- Granted: 2024-06-11
- Earliest claimed: 2021-05-28
- Assignees: Thermo Finnigan LLC
- Applicants: Thermo Finnigan LLC
- Inventors: Bennett S. Kalafut; Dhruva D. Kulkarni; Satendra Prasad; Michael W. Belford
- Web page: https://patentgrep.com/patent/US12007359B2

## CPC

- G01N 27/623: combined with mass spectrometry
- G01N 27/624: Differential mobility spectrometry [DMS]; Field asymmetric-waveform ion mobility spectrometry [FAIMS]
- H01J 49/0031: {Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence)}
- H01J 49/022: {Circuit arrangements, e.g. for generating deviation currents or voltages (regulating electric or magnetic variables in general, e.g. current, magnetic field G05F); Components associated with high voltage supply (high voltage supply per se H02M)}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H)

## Abstract

Adjusting compensation voltage (CV) parameters of an ion mobility device is described. In one instance, the CV parameters are adjusted to reflect a different CV range, a number of CV steps, or a CV step size to increase throughput of a mass spectrometer.

## Claims

### Claim 1

A mass spectrometry system comprising:
an ionization source configured to receive a sample and form ions from the sample;
a field asymmetric-waveform ion-mobility spectrometry (FAIMS) device configured to receive the ions; and
a controller configured to acquire peak information related to transmission of the ions through the FAIMS device based on initial compensation voltage (CV) parameters used for the FAIMS device, and the controller configured to adjust the initial CV parameters based on the peak information to generate adjusted CV parameters used by the FAIMS device, the adjusted CV parameters being one or more of: a CV range different than the initial CV parameters, a number of CV steps within the CV range different than the initial CV parameters, or a CV step size between CV values of the CV range different than the initial CV parameters, and wherein the adjusted CV parameters includes more steps within the CV range than the initial CV parameters.

### Claim 2 (depends on 1)

The mass spectrometry system of claim 1, wherein the adjusted CV parameters includes a narrower CV range than the initial CV parameters.

### Claim 3 (depends on 1)

The mass spectrometry system of claim 1, wherein the adjusted CV parameters includes a smaller CV step size than the initial CV parameters.

### Claim 4 (depends on 1)

The mass spectrometry system of claim 1, wherein the controller is further configured to apply a time delay based on the CV step size.

### Claim 5 (depends on 1)

The mass spectrometry system of claim 1, wherein the controller is further configured to generate a scan schedule ordering scans based on the adjusted CV parameters.

### Claim 6 (depends on 5)

The mass spectrometry system of claim 5, wherein the scan schedule is ordered based on ascending or descending CV values of each of the scans within an overlapping retention time window.

### Claim 7 (depends on 5)

The mass spectrometry system of claim 5, wherein the scan schedule includes a first scan having a first CV value within the CV range, and a second scan having a second CV value within the CV range, and the controller is configured to determine that the first CV value and the second CV value are within a threshold range of each other, and the controller is configured to modify one or both of the first CV value or the second CV value to be the same as the other.

### Claim 8

A method of operating a mass spectrometer comprising:
acquiring, by a processor, peak information indicative of transmissions of ions through an ion mobility device using first compensation voltage (CV) parameters;
determining, by the processor, second CV parameters based on the peak information, the second CV parameters being different from the first CV parameters by one or more of: a CV range, a number of CV steps within the CV range, or a CV step size between CV values of the CV range, and wherein the number of CV steps within the CV range of the second CV parameters is more than the number of CV steps of the first CV parameters;
applying the second CV parameters to the ion mobility device; and
acquiring mass spectra using the ion mobility device applied with the second CV parameters.

### Claim 9 (depends on 8)

The method of claim 8, wherein the CV range of the second CV parameters is smaller than the CV range of the first CV parameters.

### Claim 10 (depends on 8)

The method of claim 8, wherein the CV step size of the second CV parameters is smaller than the CV step size of the first CV parameters.

### Claim 11 (depends on 8)

The method of claim 8, wherein applying the second CV parameters to the ion mobility device includes applying a time delay before acquisition of the mass spectra, the time delay based on the CV step size.

### Claim 12 (depends on 8)

The method of claim 8, the method further comprising:
generating a scan schedule ordering scans based on the second CV parameters, the scan schedule ordering scans based on ascending or descending CV values of each of the scans within an overlapping retention time window, and wherein acquiring the mass spectra is based on the scan schedule.

### Claim 13 (depends on 12)

The method of claim 12, wherein the scan schedule includes a first scan having a first CV value within the CV range, and a second scan having a second CV value within the CV range, and generating the scan schedule includes:
determining that the first CV value and the second CV value are within a threshold range; and
modifying one or both of the first CV value or the second CV value to be the same as the other.

### Claim 14

A mass spectrometry system comprising:
an ion mobility device configured to transmit ions; and
a controller circuit configured to acquire transmission information indicative of the ions transmitting through the ion mobility device based on use of first compensation voltage (CV) parameters, configured to generate second CV parameters different than the first CV parameters, and configured to use the second CV parameters to facilitate the transmission of the ions through the ion mobility device, the second CV parameters being different than the first CV parameters by one or more of: a CV range, a number of CV steps within the CV range, or a CV step size among CV values used within the CV range, wherein the number of CV steps of the second CV parameters is hither than the number of CV steps for the first CV parameters.

### Claim 15 (depends on 14)

The mass spectrometry system of claim 14, wherein the CV range of the second CV parameters is narrower than the CV range of the first CV parameters.

### Claim 16 (depends on 14)

The mass spectrometry system of claim 14, wherein the CV step size of the second CV parameters is smaller than the CV step size of the first CV parameters.

### Claim 17 (depends on 14)

The mass spectrometry system of claim 14, wherein the controller circuit is further configured to acquire a mass spectrum of the ions transmitting through the ion mobility device following a time delay applied that has a magnitude based on the CV step size of the second CV parameters.

### Claim 18 (depends on 14)

The mass spectrometry system of claim 14, wherein the controller circuit is further configured to generate a scan schedule ordering scans based on the second CV parameters.

### Claim 19 (depends on 18)

The mass spectrometry system of claim 18, wherein generating the scan schedule includes:
identifying scans within the scan schedule having overlapping retention time windows;
identifying CVs of the scans identified as having overlapping retention time windows; and
reordering the scan schedule based on the identification of the CVs.

### Claim 20 (depends on 19)

The mass spectrometry system of claim 19, wherein reordering the scan schedule includes ordering the scans based on ascending or descending CV values.

### Claim 21 (depends on 19)

The mass spectrometry system of claim 19, wherein the scan schedule includes a first scan having a first CV value within the CV range, and a second scan having a second CV value within the CV range, and the controller is configured to determine that the first CV value and the second CV value are within a threshold range, and the controller is configured to modify one or both of the first CV value or the second CV value based on the determination that the first CV value and the second CV value are within the threshold range.

### Claim 22

A computer program product including one or more non-transitory computer-readable media having computer programs instructed stored therein, the computer program instructions being configured such that, when executed by one or more computing devices, the computer program instructions cause the one or more computing devices to:
acquire peak information indicative of transmissions of ions through an ion mobility device using first compensation voltage (CV) parameters;
determine second CV parameters based on the peak information, the second CV parameters being different from the first CV parameters by one or more of: a CV range, a number of CV steps within the CV range, or a CV step size between CV values of the CV range, wherein the number of CV steps of the second CV parameters is higher than the number of CV steps for the first CV parameters;
apply the second CV parameters to the ion mobility device; and
acquire mass spectra using the ion mobility device applied with the second CV parameters.