# US 12,006,543 B2 · Device preparation using condensed nucleic acid particles

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 12,006,543 B2
- Document ID: US12006543B2
- Status: Granted
- Kind: B2
- Application number: 17/217,492
- Filed: 2021-03-30
- Published: 2021-07-15
- Granted: 2024-06-11
- Earliest claimed: 2013-09-13
- Assignees: Life Technologies Corporation
- Applicants: Life Technologies Corporation
- Inventors: Guobin Luo; Marina Sedova; David Light; Ryan Jones; Mohammad Alanjary
- Web page: https://patentgrep.com/patent/US12006543B2

## CPC

- C12Q 1/6874: involving nucleic acid arrays, e.g. sequencing by hybridisation
- C12Q 1/6806: Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

A method for depositing particles on a surface includes receiving a plurality of particles, a particle of the plurality of particles having a polymer matrix conjugated to nucleic acid strands, exposing the plurality of particles to a solution; and applying the plurality of particles to a surface following exposing the plurality of particles to the solution, particles of the plurality of particles depositing on the surface. The solution includes a magnesium salt in a range of 30 mM to 500 mM, a potassium salt in a range of 0.8 M to 1.0 M, a buffering agent in a range of 150 mM to 500 mM, and a surfactant in a range of 0.05% to 0.5%.

## Claims

### Claim 1

A method for depositing particles on a surface, the method comprising:
receiving a plurality of particles, a particle of the plurality of particles having a hydrogel polymer matrix conjugated to nucleic acid strands;
exposing the plurality of particles to a solution including:
a magnesium salt in a range of 30 mM to 500 mM;
a potassium salt in a range of 0.8 M to 1.0 M;
a buffering agent in a range of 150 mM to 500 mM; and
a surfactant in a range of 0.05% to 0.5%; and
applying the plurality of particles to a surface following exposing the plurality of particles to the solution, particles of the plurality of particles depositing on the surface.

### Claim 2 (depends on 1)

The method of claim 1, wherein the nucleic acid strands condense in response to the solution to form condensed particles having a reduced diameter following exposing the plurality of particles to the solution.

### Claim 3 (depends on 1)

The method of claim 1, wherein the surface defines a plurality of wells, the particles of the plurality of particles depositing into the wells on the surface.

### Claim 4 (depends on 3)

The method of claim 3, further comprising washing the surface to remove the solution from the particles deposited in the wells, the particles expanding and remaining in the wells.

### Claim 5 (depends on 1)

The method of claim 1, wherein a pH of the solution is in a range of 7 to 9.

### Claim 6 (depends on 1)

The method of claim 1, wherein the buffering agent includes tris(hydroxymethyl)aminomethane.

### Claim 7 (depends on 1)

The method of claim 1, wherein the surfactant includes a polyethylene glycol based surfactant.

### Claim 8 (depends on 7)

The method of claim 7, wherein the polyethylene glycol based surfactant includes an octylphenyl ether of polyethylene glycol.

### Claim 9 (depends on 1)

The method of claim 1, further comprising polyethylene glycol based polymer.

### Claim 10 (depends on 9)

The method of claim 9, wherein the polyethylene glycol based polymer has a molecular weight in a range of 1000 Da to 100000 Da.

### Claim 11 (depends on 1)

The method of claim 1, wherein the polyethylene glycol based polymer is present in a range of 0.1 wt % to 20.0 wt %.

### Claim 12 (depends on 1)

The method of claim 1, wherein the magnesium salt is present in a range of 50 mM to 1M.

### Claim 13 (depends on 12)

The method of claim 12, wherein the magnesium salt is present in a range of 50 mM to 800 mM.

### Claim 14

A method for depositing particles on a surface, the method comprising:
receiving a plurality of particles, a particle of the plurality of particles having a hydrogel polymer matrix conjugated to nucleic acid strands;
exposing the plurality of particles to a solution including:
a magnesium salt in a range of 30 mM to 1M;
polyethylene glycol based polymer in a range of 0.5 wt % to 10.0 wt %;
a potassium salt in a range of 0.8 M to 1.0 M; and
a buffering agent in a range of 150 mM to 500 mM; and
applying the plurality of particles to a surface following exposing the plurality of particles to the solution, particles of the plurality of particles depositing on the surface.

### Claim 15 (depends on 14)

The method of claim 14, wherein the pH is in a range of 7 to 9.

### Claim 16 (depends on 14)

The method of claim 14, wherein the magnesium salt is present in a range of 50 mM to 1M.

### Claim 17 (depends on 16)

The method of claim 16, wherein the magnesium salt is present in a range of 50 mM to 800 mM.

### Claim 18 (depends on 14)

The method of claim 14, wherein the polyethylene glycol based polymer has a molecular weight in a range of 1000 Da to 100000 Da.

### Claim 19 (depends on 14)

The method of claim 14, wherein the polyethylene glycol based polymer is present in a range of 0.1 wt % to 20.0 wt %.

### Claim 20 (depends on 19)

The method of claim 19, wherein the polyethylene glycol based polymer is present in a range of 0.5 wt % to 15.0 wt %.