US 12,006,527 B2Granted

Coryneform bacterium transformant and method for producing 2-phenylethanol using same

AssigneesResearch Institute of Innovative Technology for the Earth, Sumitomo Bakelite Co., Ltd.

InventorsMasayuki Inui, Kazumi Hiraga, Masako Suda, Masayoshi Hashizume, Junya Ishida

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Abstract

Provided is a microorganism that is able to produce 2-phenylethanol at a high concentration, and a method of efficiently producing 2-phenylethanol by using a saccharide as a raw material. Provided is a coryneform bacterium transformant in which a shikimate pathway is activated, and further, a gene that encodes an enzyme having phenylpyruvate decarboxylase activity is introduced in such a manner that the gene can be expressed. Also provided is a 2-phenylethanol producing method that includes causing the coryneform bacterium transformant according to the present disclosure to react in water containing a saccharide.

Claims23

Dependent claims

A coryneform bacterium transformant selected from the group consisting of:

(a) a Corynebacterium glutamicum transformant obtained by introducing a gene that encodes an enzyme having phenylpyruvate decarboxylase activity into a Corynebacterium glutanicum in such a manner that the gene can be expressed, wherein the gene that encodes an enzyme having phenylpyruvate decarboxylase activity is a gene comprising a sequence having at least 98% identity to SEQ ID NO:
2;
(b) a coryneform bacterium transformant obtained by introducing a plasmid having a gene that encodes an enzyme having phenylpyruvate decarboxylase activity into a coryneform bacterium in such a manner that the gene can be expressed, wherein the gene that encodes an enzyme having phenylpyruvate decarboxylase activity is a gene comprising a sequence having at least 98% identity to SEQ ID NO:
2;
and
(c) a coryneform bacterium transformant obtained by introducing a gene that encodes an enzyme having phenylpyruvate decarboxylase activity into a coryneform bacterium in such a manner that the gene can be expressed, wherein the gene that encodes an enzyme having phenylpyruvate decarboxylase activity is a gene comprising the sequence of SEQ ID NO:3.

3 more elements

Dependencies are inferred from the claim text.