# US 12,006,496 B2 · Isotachophoresis for purification of nucleic acids > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,006,496 B2 - Document ID: US12006496B2 - Status: Granted - Kind: B2 - Application number: 18/141,641 - Filed: 2023-05-01 - Published: 2023-11-09 - Granted: 2024-06-11 - Earliest claimed: 2016-01-29 - Assignees: Purigen Biosystems, Inc. - Applicants: Purigen Biosystems, Inc. - Inventors: Lewis A. Marshall; Amy L. Hiddessen; Nathan P. Hoverter; Klint A. Rose; Juan G. Santiago - Web page: https://patentgrep.com/patent/US12006496B2 ## CPC - C12N 15/101: {by chromatography, e.g. electrophoresis, ion-exchange, reverse phase} - B01L 3/502753: {characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation} - C12N 15/10: Processes for the isolation, preparation or purification of DNA or RNA (chemical preparation of DNA or RNA C07H21/00; preparation of non-structural polynucleotides from microorganisms or with enzymes C12P19/34) - C12Q 1/6806: Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) - G01N 27/4473: {by electric means} - G01N 27/44739: {Collecting the separated zones, e.g. blotting to a membrane or punching of gel spots} - G01N 27/44791: {Microapparatus (sample containers with integrated microfluidic structures B01L3/5027)} - G01N 27/44795: {Isoelectric focusing} - B01L 3/502738: {characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746)} - B01L 2300/0627: Sensor or part of a sensor is integrated - B01L 2300/0867: Multiple inlets and one sample wells, e.g. mixing, dilution - B81B 1/004: {Through-holes, i.e. extending from one face to the other face of the wafer} - B81B 7/0087: {On-device systems and sensors for controlling, regulating or monitoring} - B81B 2201/0278: Temperature sensors - B81B 2201/05: Microfluidics - B81B 2203/0338: Channels - G01N 27/44704: {Details; Accessories} - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - B81B: MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES (piezoelectric, electrostrictive or magnetostrictive elements per se H10N30/00, H10N35/00) ## Abstract The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing. ## Claims ### Claim 1 A kit comprising: (a) microfluidic chip comprising two or more fluidic channels each in fluid communication with a sample reservoir configured to receive a sample, a first buffer reservoir configured to receive a buffer, a second buffer reservoir configured to receive a second buffer, a third buffer reservoir configured to receive a third buffer and an elution reservoir configured to receive an eluate; and (b) one or more buffers. ### Claim 2 (depends on 1) The kit according to claim 1, wherein the one or more buffers comprises a leading electrolyte buffer. ### Claim 3 (depends on 1) The kit according to claim 1, wherein the one or more buffers comprises a trailing electrolyte buffer. ### Claim 4 (depends on 1) The kit according to claim 1, wherein the one or more buffers comprises a sample buffer. ### Claim 5 (depends on 1) The kit according to claim 1, wherein the one or more buffers comprises a lysis buffer. ### Claim 6 (depends on 1) The kit according to claim 1, wherein the one or more buffers comprises a leading electrolyte buffer, a trailing electrolyte buffer, a sample buffer and a lysis buffer. ### Claim 7 (depends on 6) The kit according to claim 6, wherein the kit further comprises one or more enzymes. ### Claim 8 (depends on 7) The kit according to claim 7, wherein the one or more enzymes comprises one or more of an RNase, a DNase and a proteinase. ### Claim 9 (depends on 1) The kit according to claim 1, wherein the two or more fluidic channels each comprise a branched fluidic channel comprising a loading section and an isotachophoresis (“ITP”) section wherein the ITP section comprises a leading electrolyte branch and an elution branch, meeting at a branch point. ### Claim 10 (depends on 9) The kit according to claim 9, wherein the loading section comprises: (1) a trailing electrolyte well fluidically connected to a sample input region of the fluidic channel; and (2) a sample input well fluidically connected to the sample input region of the fluidic channel. ### Claim 11 (depends on 10) The kit according to claim 10, wherein the each fluidic channel further comprises a capillary barrier positioned between the sample input region of the fluidic channel and the ITP section. ### Claim 12 (depends on 10) The kit according to claim 10, wherein the loading section further comprises a trailing capillary barrier positioned between the trailing electrolyte well and the sample input well, and a gas channel connected to the trailing capillary barrier and to a pneumatic pressure port. ### Claim 13 (depends on 9) The kit according to claim 9, wherein the leading electrolyte branch comprises a leading electrolyte well fluidically connected to the fluidic channel. ### Claim 14 (depends on 9) The kit according to claim 9, wherein the elution branch comprises a sample elution well fluidically connected to the fluidic channel. ### Claim 15 (depends on 9) The kit according to claim 9, wherein the ITP section further comprises a leading electrolyte buffering well fluidically connected to the leading electrolyte well through a leading electrolyte buffering channel. ### Claim 16 (depends on 15) The kit according to claim 15, wherein the leading electrolyte buffering channel comprises a leading capillary barrier and a gas channel connected to the leading capillary barrier and to a pneumatic pressure port. ### Claim 17 (depends on 9) The kit according to claim 9, wherein the elution branch further comprises an elution buffering well fluidically connected to the elution well through an elution buffering channel. ### Claim 18 (depends on 17) The kit according to claim 17, wherein the elution buffering channel comprises an elution capillary barrier, and a gas channel connected to the elution capillary barrier and to a pneumatic pressure port. ### Claim 19 (depends on 17) The kit according to claim 17, wherein the elution buffering channel comprises an elution capillary barrier, and a gas channel connected to the elution capillary barrier and to a pneumatic pressure port. ### Claim 20 (depends on 1) The kit according to claim 1, wherein the kit comprises 8 fluidic channels.