# US 12,006,458 B2 · Complex and detection device

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- Number: US 12,006,458 B2
- Document ID: US12006458B2
- Status: Granted
- Kind: B2
- Application number: 17/118,652
- Filed: 2020-12-11
- Published: 2021-04-01
- Granted: 2024-06-11
- Earliest claimed: 2018-11-30
- Assignees: Panasonic Intellectual Property Management Co., Ltd.
- Applicants: Panasonic Intellectual Property Management Co., Ltd.
- Inventors: Hiroto Yanagawa
- Web page: https://patentgrep.com/patent/US12006458B2

## CPC

- C09K 11/025: {non-luminescent particle coatings or suspension media}
- C09K 11/59: containing silicon
- C01B 33/02: Silicon (forming single crystals or homogeneous polycrystalline material with defined structure C30B)
- C09K 11/02: Use of particular materials as binders, particle coatings or suspension media therefor
- G01N 21/6428: {Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53)}
- G01N 21/648: {using evanescent coupling or surface plasmon coupling for the excitation of fluorescence}
- G01N 33/543: with an insoluble carrier for immobilising immunochemicals
- B82Y 15/00: Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
- B82Y 30/00: Nanotechnology for materials or surface science, e.g. nanocomposites
- B82Y 40/00: Manufacture or treatment of nanostructures
- G01N 2021/1727: {Magnetomodulation}
- G01N 2021/258: {Surface plasmon spectroscopy, e.g. micro- or nanoparticles in suspension}
- C09K: MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C01B: NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; {METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES

## Abstract

The present disclosure provides a complex having satisfactory dispersibility and stable luminescence characteristics. A complex of the present disclosure includes: a first substance having a property of specifically binding to a target substance; a quantum dot that contains silicon as a main component and that is negatively charged on a surface; and a linker substance that contains a compound represented by general formula (1) below and that covers the surface of the quantum dot, where the first substance has been immobilized on the surface of the quantum dot through the linker substance: X—L—Si—(R1)(R2)(OR3) (1) where X is a basic functional group; R1, R2, and R3 are each independently an alkyl group; and L is an alkylene group.

## Claims

### Claim 1

A complex comprising:
a first substance having a property of specifically binding to a target substance;
a quantum dot that contains silicon as a main component and that is negatively charged on a surface; and
a linker substance that contains a compound represented by general formula (1) below and that covers the surface of the quantum dot,
wherein:
the first substance has been immobilized on the surface of the quantum dot through the linker substance:
X—L—Si—(R1)(R2)(OR3) (1)
where
X is a basic functional group
R1, R2, and R3 are each independently an alkyl group, and
L is an alkylene group, and
the linker substance has one silanol group bonding to the silicon quantum dot and the first substance bonds to the linker substance through the basic functional group.

### Claim 2 (depends on 1)

The complex according to claim 1, wherein
X is an amino group.

### Claim 3 (depends on 2)

The complex according to claim 2, wherein
R1 and R2 are a methyl group,
R3 is an ethyl group, and
L is a propylene group.

### Claim 4 (depends on 1)

The complex according to claim 1, wherein the surface of the quantum dot contains boron and phosphorus.