# US 12,006,315 B2 · Intermediate useful for the synthesis of TGF-beta inhibitors and a method of preparing TGF-beta inhibitors using the same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,006,315 B2 - Document ID: US12006315B2 - Status: Granted - Kind: B2 - Application number: 18/188,912 - Filed: 2023-03-23 - Published: 2023-09-28 - Granted: 2024-06-11 - Earliest claimed: 2022-03-25 - Assignees: Medpacto Inc. - Applicants: Medpacto Inc. - Inventors: Seung Ho Lee - Web page: https://patentgrep.com/patent/US12006315B2 ## CPC - C07D 471/04: Ortho-condensed systems - C07D: HETEROCYCLIC COMPOUNDS (macromolecular compounds C08) ## Abstract The present invention provides an intermediate for synthesizing a TGF-β inhibitor represented by Chemical Formula 1 and an improved method for preparing the TGF-β inhibitor represented by Chemical Formula 1 using the same. The preparation method according to the present invention can not only allow inexpensive and safe reagents to be used, but also simplify the synthesis steps and purification methods to improve the reaction yield, thereby maximizing the production efficiency of the TGF-β inhibitor represented by Chemical Formula 1 to be used usefully for mass production. ## Claims ### Claim 1 A method for preparing a compound of Chemical Formula 1, the method comprising: obtaining a compound of Chemical Formula 4 by reacting a compound of Chemical Formula 2 with a compound of Chemical Formula 3, and obtaining a compound of Chemical Formula 1 by deprotecting the compound of Chemical Formula 4: wherein, in the formulae, X and Y are each independently halogen; PG is a protecting group selected from the group consisting of butoxycarbonyl (Boc), benzyloxycarbonyl (Cbz), 9-fluorenylmethoxycarbonyl (Fmoc), acetyl, benzoyl and tosyl; Ra is independently hydrogen, halogen, C1-6 alkyl, C1-6 haloalkyl, C3-6 cycloalkyl, hydroxyl, —O-C1-6 alkyl, —O-C1-6 haloalkyl, —O-C3-6 cycloalkyl, amino, —NH-C1-6 alkyl, —NH-C1-6 haloalkyl, —NH-C3-6 cycloalkyl, —S-C1-6 alkyl, —S-C1-6 haloalkyl, —S-C3-6 cycloalkyl, cyano, or nitro; and m is 0, 1, 2, 3 or 4. ### Claim 2 (depends on 1) The method of claim 1, wherein the compound of Chemical Formula 1 is a compound of Chemical Formula 1a: ### Claim 3 (depends on 1) The method of claim 1, wherein the compound of Chemical Formula 2 is a compound of Chemical Formula 2a: ### Claim 4 (depends on 1) The method of claim 1, wherein the compound of Chemical Formula 3 is a compound of Chemical Formula 3a: ### Claim 5 (depends on 1) The method of claim 1, wherein the compound of Chemical Formula 4 is a compound of Chemical Formula 4a: ### Claim 6 (depends on 1) The method of claim 1, wherein the obtaining of the compound of Chemical Formula 4 by reacting the compound of Chemical Formula 2 with the compound of Chemical Formula 3 comprises coupling an amine of the compound of Chemical Formula 3 to a position of an X substituent of the compound of Chemical Formula 2 in the presence of a base, and then forming imidazole by a dehydration reaction. ### Claim 7 (depends on 6) The method of claim 6, wherein the base is selected from the group consisting of potassium bicarbonate, potassium carbonate, potassium phosphate, sodium acetate, 1,4-diazabicyclo[2.2.2]octane (DABCO), and triethylamine. ### Claim 8 (depends on 6) The method of claim 6, wherein in the reaction, dimethylformamide (N,N-dimethylformamide, DMF), acetonitrile, tetrahydrofuran (THF), toluene or a mixture thereof is used as a reaction solvent. ### Claim 9 (depends on 6) The method of claim 6, wherein the reaction further comprises a desiccant to prevent side reactions, and the desiccant is selected from among molecular sieves, sodium sulfate and magnesium sulfate. ### Claim 10 (depends on 6) The method of claim 6, wherein the reaction is performed at 20° C. to 95° C. ### Claim 11 (depends on 1) The method of claim 1, wherein in the obtaining of the compound of Chemical Formula 1 by deprotecting the compound of Chemical Formula 4, dichloromethane, ethylacetate, methanol or a mixture thereof is used as a reaction solvent. ### Claim 12 (depends on 1) The method of claim 1, wherein the compound of Chemical Formula 2 is obtained by converting an Rb substituent of a compound of Chemical Formula 5 into halogen, in the formula, Ra is independently hydrogen, halogen, C1-6 alkyl, C1-6 haloalkyl, C3-6 cycloalkyl, hydroxyl, —O-C1-6 alkyl, —O-C1-6 haloalkyl, —O-C3-6 cycloalkyl, amino, —NH-C1-6 alkyl, —NH-C1-6 haloalkyl, —NH-C3-6 cycloalkyl, —S-C1-6 alkyl, —S-C1-6 haloalkyl, —S-C3-6 cycloalkyl, cyano, or nitro; m is 0, 1, 2, 3 or 4; and Rb is hydrogen, hydroxyl, cyano, nitro, or amino. ### Claim 13 (depends on 12) The method of claim 12, wherein the compound of Chemical Formula 5 is a compound of Chemical Formula 5a: ### Claim 14 (depends on 1) The method of claim 1, wherein the compound of Chemical Formula 3 is obtained by forming an amidine through a Pinner reaction of a nitrile in Chemical Formula 6, in the formula, Y is halogen; and PG is a protecting group selected from the group consisting of butoxycarbonyl (Boc), benzyloxycarbonyl (Cbz), 9-fluorenylmethoxycarbonyl (Fmoc), acetyl, benzoyl and tosyl. ### Claim 15 (depends on 14) The method of claim 14, wherein the Pinner reaction comprises forming an iminoester salt by reacting the compound of Chemical Formula 6 with an alkoxy, and obtaining a compound of Chemical Formula 3 by reacting the iminoester salt with an ammonium salt. ### Claim 16 (depends on 14) The method of claim 14, wherein the compound of Chemical Formula 6 is obtained by introducing a protecting group into an amine of a compound of Chemical Formula 7, in the formula, Y is a halogen. ### Claim 17 (depends on 16) The method of claim 16, wherein the compound of Chemical Formula 7 is obtained by introducing acetonitrile into an amine of a compound of Chemical Formula 8 in the presence of a base, in the formula, Y is halogen. ### Claim 18 A compound represented by Chemical Formula 4: in the formula, Y is halogen; PG is a protecting group selected from the group consisting of butoxycarbonyl (Boc), benzyloxycarbonyl (Cbz), 9-fluorenylmethoxycarbonyl (Fmoc), acetyl, benzoyl and tosyl; Ra is independently hydrogen, halogen, C1-6 alkyl, C1-6 haloalkyl, C3-6 cycloalkyl, hydroxyl, —O-C1-6 alkyl, —O-C1-6 haloalkyl, —O-C3-6 cycloalkyl, amino, —NH-C1-6 alkyl, —NH-C1-6 haloalkyl, —NH-C3-6 cycloalkyl, —S-C1-6 alkyl, —S-C1-6 haloalkyl, —S-C3-6 cycloalkyl, cyano, or nitro; and m is 0, 1, 2, 3 or 4. ### Claim 19 (depends on 18) The compound of claim 18, wherein the compound is represented by Chemical Formula 4a: