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US 12,005,452 B2Granted
Nanoscale biochemical sample preparation and analysis
AssigneeBattelle Memorial Institute
InventorsRyan T. Kelly, Ying Zhu, Richard D. Smith
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Abstract
Provided herein are methods and systems for biochemical analysis, including compositions and methods for processing and analysis of small cell populations and biological samples (e.g., a robotically controlled chip-based nanodroplet platform). In particular aspects, the methods described herein can reduce total processing volumes from conventional volumes to nanoliter volumes within a single reactor vessel (e.g., within a single droplet reactor) while minimizing losses, such as due to sample evaporation.
Claims6
A platform for biological sample preparation, comprising:
- a substrate comprising at least one reactor vessel having one or more hydrophilic surfaces configured for containment of a biological sample, wherein the hydrophilic surface is formed on an upper surface of a pillar and defines the lateral boundary of the least one reactor vessel and the hydrophilic surfaces have a non-zero total surface area less than 25 mm2;
- a spacer containing an aperture, wherein the aperture is dimensioned to surround the at least one reactor vessel when the spacer is positioned on the substrate;
- a cover positioned on the spacer;
- and
- a membrane interposed between the spacer and the cover and the membrane overlies the spacer, wherein the membrane has a membrane aperture that is dimensioned to surround the at least one reactor vessel when the membrane is positioned on the spacer, wherein the substrate, spacer and cover are each formed from a material that is transparent to optical light.
2 more elements
Dependencies are inferred from the claim text.