# US 12,005,436 B2 · Devices and methods for separating particles from a liquid > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,005,436 B2 - Document ID: US12005436B2 - Status: Granted - Kind: B2 - Application number: 16/615,877 - Filed: 2018-05-25 - Published: 2020-04-16 - Granted: 2024-06-11 - Earliest claimed: 2017-05-26 - Assignees: StemCell Technologies Inc. - Applicants: Stemcell Technologies Inc. - Inventors: Eric Jervis; Tom Glawdel - Web page: https://patentgrep.com/patent/US12005436B2 ## CPC - B01L 3/502: {with fluid transport, e.g. in multi-compartment structures} - B01L 3/5082: {Test tubes per se} - C12M 47/02: {Separating microorganisms from the culture medium; Concentration of biomass (separating microorganisms from their culture media C12N1/02)} - B01L 3/502746: {characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves} - B01L 2200/0668: Trapping microscopic beads - B01L 2300/0858: Side walls - B01L 2400/0406: capillary forces - B01L 2400/086: using baffles or other fixed flow obstructions - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract Surfaces contactable by a bulk liquid for use in particle isolation are described. The surfaces comprise a plurality of ribs spaced apart to capillarily retain a portion of the bulk liquid and particles of interest therebetween. Collectively, the portion of the bulk liquid retained by the plurality of ribs on the surface forms a liquid film. The one or more particles received within the space and enveloped by the liquid film may be protected from one or more forces exerted by a draining meniscus passing over the surface. ## Claims ### Claim 1 An apparatus for separating particles from a bulk liquid, the apparatus comprising: a container having: a surface to be contacted by a particle-containing bulk liquid, a plurality of ribs on the surface, each rib of the plurality of ribs being positioned between and spaced apart from two adjacent ribs on the surface by a substantially uniform pitch distance; and a space between each rib and each of the two adjacent ribs being dimensioned to capillarily retain therebetween a portion of the bulk liquid and at least a portion of the particles entrained therein when the liquid contacting the surface is removed away from the surface, wherein the surface is an inner sidewall of the container extending from an upper end having an opening to a bottom wall opposed to the opening, and the plurality of ribs extend away from the inner sidewall toward an interior of the container, and wherein the container is a tube, and wherein the plurality of ribs extend in a direction between the opening and the opposed bottom wall along the inner sidewall and are distributed around an inner perimeter of the sidewall of the tube and the pitch distance is less than about 1 mm. ### Claim 2 (depends on 1) The apparatus according to claim 1, wherein a first rib extends along a first longitudinal axis, and wherein a second rib extends along a second longitudinal axis. ### Claim 3 (depends on 2) The apparatus according to claim 2, wherein the second longitudinal axis is substantially parallel to the first longitudinal axis. ### Claim 4 (depends on 3) The apparatus according to claim 3, wherein the first longitudinal axis and the second longitudinal axis are generally linear. ### Claim 5 (depends on 1) The apparatus according to claim 1, wherein a first rib includes a first sidewall extending away from the surface and having a first base edge and a first protruding edge, and a second sidewall extending away from the surface and having a second base edge and a second protruding edge, the first base edge spaced apart from the second base edge by a first rib width and the first protruding edge connected to the second protruding edge at a first apex height, and wherein a second rib includes a third sidewall extending away from the surface and having a third base edge and a third protruding edge, and a fourth sidewall extending away from the surface and having a fourth base edge and a fourth protruding edge, the third base edge spaced apart from the fourth base edge by a second rib width and the third protruding edge connected to the fourth protruding edge at a second apex height. ### Claim 6 (depends on 5) The apparatus according to claim 5, wherein the first apex height and the second apex height are each between about 20 um to about 1 mm. ### Claim 7 (depends on 5) The apparatus according to claim 5, further comprising a third rib spaced apart from the second rib by the pitch distance, the third rib including a fifth sidewall extending away from the surface and having a fifth base edge and a fifth protruding edge, and a sixth sidewall extending away from the surface and having a sixth base edge and a sixth protruding edge, the fifth base edge spaced apart from the sixth base edge by a third rib width and the fifth protruding edge connected to the sixth protruding edge at a third apex height. ### Claim 8 (depends on 7) The apparatus according to claim 7, wherein the third apex height is between about 20 um and about 1 mm and is different than the first and second apex heights. ### Claim 9 (depends on 7) The apparatus according to claim 7, wherein the first protruding edge is connected to the second protruding edge by a first top wall, and the third protruding edge is connected to the fourth protruding edge by a second top wall. ### Claim 10 (depends on 9) The apparatus according to claim 9, wherein the fifth protruding edge is connected to the sixth protruding edge by a third top wall. ### Claim 11 (depends on 10) The apparatus according to claim 10, wherein a width of the first top wall, the second top wall and the third top wall is between about 1 um and about 1 mm. ### Claim 12 (depends on 1) The apparatus according to claim 1, wherein the pitch distance is at least 1 um. ### Claim 13 (depends on 5) The apparatus according to claim 5, wherein the first rib has a first cross-sectional shape taken in a plane orthogonal to the first longitudinal axis and the second rib has a second cross-sectional shape taken in the plane. ### Claim 14 (depends on 13) The apparatus according to claim 13, wherein the first cross-sectional shape is the same as the second cross-sectional shape. ### Claim 15 (depends on 13) The apparatus according to claim 13, wherein the first cross-sectional shape is a quadrilateral. ### Claim 16 (depends on 13) The apparatus according to claim 13, wherein the first cross-sectional shape is a triangle. ### Claim 17 (depends on 9) The apparatus according to claim 9, wherein the first top wall and the second top wall are coplanar with the inner surface of the container. ### Claim 18 (depends on 2) The apparatus according to claim 2, wherein the first and second ribs extend a rib length along a surface longitudinal axis, and wherein the rib length is between 5% and 95% of the surface longitudinal axis. ### Claim 19 A container for holding a particle-containing bulk liquid, the container comprising: a closed bottom end having a bottom wall, an open upper end, one or more sidewalls extending from the bottom wall to the upper end along a longitudinal axis in a direction from the open upper end to the closed bottom end, and an inner surface bounding an interior of the container and an opposed outer surface; a plurality of ribs on the inner surface, the plurality of ribs comprising a pair of end ribs extending parallel to opposed edges of the inner surface and a plurality of intervening ribs flanked by the pair of end ribs, wherein each rib of the plurality of intervening ribs (i) is spaced apart from two adjacent ribs by a substantially uniform pitch distance, (ii) extends in a direction along the longitudinal axis, and (iii) extends away from the inner surface toward an interior of the container; and a substantially uniform space between adjacent ribs of the plurality of ribs, whereby when bulk liquid is contained in the interior of the container the bulk liquid contacts the inner surface, the plurality of ribs, and the spaces there, and whereby the plurality of ribs are dimensioned to capillarily-retain therebetween a portion of the bulk liquid and at least a portion of the particles entrained therein when the bulk liquid contacting the surface is removed away from the surface. ### Claim 20 A method for separating particles from a particle-containing bulk liquid in a container having a surface that is an inner sidewall extending from an upper end having an opening to a bottom wall opposed to the opening, a plurality of ribs extending along the inner sidewall and away from the inner sidewall toward an interior of the container, each rib of the plurality of ribs being positioned between and spaced apart from two adjacent ribs on the surface by a uniform pitch distance sufficient to receive a diameter of at least one particle, and a space between the first rib and the second rib, the method comprising: contacting the inner sidewall with the particle-containing bulk liquid, whereby the particle-containing bulk liquid contacts the surface, the first and second ribs, and the space between the first and second ribs; receiving at least a portion of the particles in the particle-containing bulk liquid into the space between the first and second ribs; removing, by pour-off, pipetting or aspiration, the particle-containing bulk liquid away from the surface in a direction along the axis in which each of the plurality of ribs extend, a portion of the particle-containing bulk liquid received in the space being capillarily-retained in the space between the first and second ribs to form a liquid film on the inner sidewall; shielding the particles of the portion of the particle-containing bulk liquid retained in the space between the first and second ribs by being entrained in the liquid film from one or more forces of a draining meniscus as residual bulk liquid and any residual particles therein is removed away from the surface; and resuspending in a buffer the shielded particles entrained within the liquid film, wherein the container is a tube comprising one sidewall and the plurality of ribs extend in a direction between the opening and the opposed bottom wall along the sidewall.