# US 12,005,433 B2 · Wash through pipettor

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- Number: US 12,005,433 B2
- Document ID: US12005433B2
- Status: Granted
- Kind: B2
- Application number: 17/327,977
- Filed: 2021-05-24
- Published: 2021-09-09
- Granted: 2024-06-11
- Earliest claimed: 2013-03-15
- Assignees: LGC Genomics Limited
- Applicants: Douglas Scientific, LLC
- Inventors: Richard J. Schoeneck; Paul H. Wagner
- Web page: https://patentgrep.com/patent/US12005433B2

## CPC

- B01L 3/0217: {of the plunger pump type}
- B01L 3/0234: {Repeating pipettes, i.e. for dispensing multiple doses from a single charge}
- B08B 3/12: by sonic or ultrasonic vibrations
- B08B 9/023: Cleaning the external surfaces {(B08B9/021 takes precedence)}
- B08B 9/0323: {Arrangements specially designed for simultaneous and parallel cleaning of a plurality of conduits}
- G01N 35/1004: {Cleaning sample transfer devices}
- G01N 35/1065: {Multiple transfer devices}
- B01L 2200/0689: Sealing
- B01L 2200/141: Preventing contamination, tampering
- B01L 2300/0829: Multi-well plates; Microtitration plates
- B01L 2300/0861: Configuration of multiple channels and/or chambers in a single devices
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B08B: CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A pipettor includes a wash chamber between an upper plate, a lower plate, an upper seal, and a lower seal. The upper and lower seal retain each other and are located between the upper and lower plates. The pipettor includes a gasket located below the lower plate, a pipette tip retained by the gasket, and a piston with a tapered tip that passes through the plates, seals, and gasket into the pipette tip. The upper and lower seal isolate the piston from the wash chamber. The pipettor includes a channel defined by the piston, extending into the pipette tip, and an actuator that advances and retracts the piston. When the actuator retracts the piston such that the piston loses sealing contact with the lower seal and the wash chamber is supplied with wash fluid and pressurized, wash fluid passes through the groove in the upper seal and into the channel.

## Claims

### Claim 1

A pipettor comprising an actuator assembly and a piston assembly, the actuator assembly comprising:
an actuator frame;
linear bearings within and connected to the actuator frame;
an actuator plate moveable on and along the linear bearings;
a ball screw;
a ball screw nut connected to the ball screw; and
an actuator motor positioned on a top end of the actuator frame, wherein the actuator motor is configured to turn the ball screw to drive the ball screw nut that is connected to the actuator plate to move the actuator plate along the linear bearings,
the piston assembly comprising:
a top seal plate;
a cylinder block;
a wash plate including a wash chamber, the wash chamber defined by a space between the top seal plate and the cylinder block suitable for containing wash fluid;
a piston plate coupled to the actuator plate;
a plurality of channels that pass through the top seal plate and the cylinder block;
a plurality of pistons positioned in and movable in respective channels of the plurality of channels, a first end of each of the plurality of pistons coupled to the piston plate; and
an upper seal and a lower seal configured to isolate the plurality of pistons from the wash chamber,
wherein the actuator assembly is coupled to the piston assembly for advancing and retracting the plurality of pistons in the respective channels, and wherein the plurality of channels is fluidly coupled with the wash chamber such that retraction of the plurality of pistons causes the pistons to lose sealing contact with the lower seal.

### Claim 2 (depends on 1)

The pipettor of claim 1, wherein a top surface of the piston plate is attached to a bottom surface of the actuator plate.

### Claim 3 (depends on 1)

The pipettor of claim 1, wherein the piston assembly further comprises:
a supply port formed in the wash plate; and
wherein the wash plate is in contact with the top seal plate via the upper seal and is in contact with the cylinder block via the lower seal.

### Claim 4 (depends on 1)

The pipettor of claim 1, wherein the upper seal and the lower seal form seals around the plurality of pistons.

### Claim 5 (depends on 1)

The pipettor of claim 1, wherein the piston assembly further comprises:
a thru tip gasket on a bottom side of the cylinder block; and
a tip gasket retainer which retains the thru tip gasket against the cylinder block.

### Claim 6 (depends on 5)

The pipettor of claim 5, further comprising a tip tray clamp and a tip tray in the tip tray clamp, the tip tray comprising:
a tip holder; and
a plurality of pipette tips extending through the tip holder,
wherein the tip tray clamp presses the tip tray against the thru tip gasket.

### Claim 7 (depends on 6)

The pipettor of claim 6, wherein the thru tip gasket is sealed against a top rim of each of the plurality of pipette tips.

### Claim 8 (depends on 1)

The pipettor of claim 1, wherein the plurality of pistons have tapered tips.

### Claim 9 (depends on 1)

The pipettor of claim 1, wherein the upper seal has a groove.