# US 12,005,424 B2 · Separation matrix and method of separation

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- Number: US 12,005,424 B2
- Document ID: US12005424B2
- Status: Granted
- Kind: B2
- Application number: 17/045,779
- Filed: 2019-04-24
- Published: 2021-03-04
- Granted: 2024-06-11
- Earliest claimed: 2018-04-25
- Assignees: Cytiva BioProcess R&D AB
- Applicants: Cytiva BioProcess R&D AB
- Inventors: Jonas Bengtsson; Linn Carlsson; Andreas Axen; Ronnie Palmgren
- Web page: https://patentgrep.com/patent/US12005424B2

## CPC

- B01J 20/267: {Cross-linked polymers}
- B01D 15/08: Selective adsorption, e.g. chromatography
- B01J 13/0052: {Preparation of gels}
- B01J 20/24: Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
- B01J 20/28023: {Fibres or filaments (fibres or filaments in the form of membranes B01J20/28038; B01J20/28007 takes precedence)}
- B01J 20/28047: {Gels}
- B01J 20/285: based on polymers
- B01J 20/291: Gel sorbents
- B01J 20/3042: {Use of binding agents; addition of materials ameliorating the mechanical properties of the produced sorbent}
- C08B 37/0039: {Agar; Agarose, i.e. D-galactose, 3,6-anhydro-D-galactose, methylated, sulfated, e.g. from the red algae Gelidium and Gracilaria; Agaropectin; Derivatives thereof, e.g. Sepharose, i.e. crosslinked agarose}
- C08L 1/02: Cellulose; Modified cellulose
- C08L 5/12: {Agar or} agar-agar {, i.e. mixture of agarose and agaropectin}; Derivatives thereof
- D21H 11/18: Highly hydrated, swollen or fibrillatable fibres
- B01J 2220/445: comprising a mixture of polymers
- B01J 2220/54: Sorbents specially adapted for analytical or investigative chromatography
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28)
- C08B: POLYSACCHARIDES; DERIVATIVES THEREOF (polysaccharides containing less than six saccharide radicals attached to each other by glycosidic linkages C07H; fermentation or enzyme-using processes C12P19/00; sugar industry C13; production of cellulose D21)
- C08L: COMPOSITIONS OF MACROMOLECULAR COMPOUNDS (compositions based on polymerisable monomers C08F, C08G; artificial filaments or fibres D01F; textile treating compositions D06)
- D21H: PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR

## Abstract

The invention discloses a separation matrix comprising polysaccharide gel beads, wherein said polysaccharide gel beads comprise embedded fibers. The invention further discloses a method of preparing the separation matrix and use of the matrix for separation purposes.

## Claims

### Claim 1

A separation matrix comprising polysaccharide gel beads, wherein said polysaccharide gel beads comprise embedded fibers, wherein the embedded fibers comprise microfibrillated cellulose, and wherein the embedded fibers are chemically cross-linked to the polysaccharide wherein the polysaccharide gel beads comprise agarose or agar.

### Claim 2 (depends on 1)

The separation matrix of claim 1, wherein said embedded fibers are dispersed in the polysaccharide gel beads.

### Claim 3 (depends on 1)

The separation matrix of claim 1, wherein the separation matrix comprises embedded fibers of up to 80% of the non-fiber matrix dry weight.

### Claim 4 (depends on 1)

The separation matrix of claim 1, wherein the embedded fibers are furcated.

### Claim 5 (depends on 1)

The separation matrix of claim 1, wherein the polysaccharide gel beads comprise agarose or agar.

### Claim 6 (depends on 1)

The separation matrix of claim 1, comprising polysaccharide gel beads with sphericity greater than 0.95 wherein the polysaccharide gel beads comprise agarose or agar.

### Claim 7

A method for preparing a separation matrix, which method comprises the steps of
a. mixing an aqueous solution of at least one gellable polysaccharide with fibers, wherein the fibers comprises microfibrillated cellulose;
b. forming gel beads of said aqueous solution;
c. cross-linking said gellable polysaccharide with said fibers to embed the fibers in the gel beads.

### Claim 8 (depends on 7)

The method of claim 7, further comprising, after step b., a step c. of cross-linking said gellable polysaccharide of said gel beads.

### Claim 9 (depends on 7)

The method according to claim 7, further comprising a step of attaching chromatography ligands to reactive hydroxyl groups of said gel beads.

### Claim 10 (depends on 7)

The method according to claim 7, wherein the solution of the gellable polysaccharide with the fibers is emulsified in an oil phase and said gel beads are formed by reducing the temperature to induce gelation.

### Claim 11 (depends on 7)

The method according to claim 7, comprising a cross-linking step which comprises addition of epichlorohydrin.

### Claim 12 (depends on 7)

The method according to claim 7, wherein the gellable polysaccharide solution comprises embedded fibers of up to 80% of non-fiber matrix dry weight.

### Claim 13 (depends on 1)

A method of use of a separation matrix, comprising using the separation matrix as defined in claim 1 to purify, isolate, or remove a target compound.

### Claim 14 (depends on 13)

The use according to claim 13 wherein said separation matrix is packed in a chromatography column.

### Claim 15 (depends on 1)

A method of separating a target compound, comprising a step of contacting the separation matrix of claim 1 with a liquid comprising said target compound.