# US 12,005,157 B2 · Isolation and co-transplantation of bone marrow mesenchymal stem cells with CD34+ hematopoietic stem/progenitor cells

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- Number: US 12,005,157 B2
- Document ID: US12005157B2
- Status: Granted
- Kind: B2
- Application number: 14/625,168
- Filed: 2015-02-18
- Published: 2015-08-20
- Granted: 2024-06-11
- Earliest claimed: 2014-02-18
- Assignees: Northwestern University; Ann & Robert H. Lurie Children's Hospital of Chicago
- Applicants: Northwestern University; Ann & Robert H. Lurie Children's Hospital of Chicago
- Inventors: Arun Sharma; Natalie J. Fuller; Matthew I. Bury; Earl Y. Cheng
- Web page: https://patentgrep.com/patent/US12005157B2

## CPC

- A61L 27/3834: {Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells}
- A61L 27/54: Biologically active materials, e.g. therapeutic substances {(A61L27/227 takes precedence)}
- A61L 27/58: Materials at least partially resorbable by the body
- A61L 2300/64: Animal cells
- A61L 2430/22: for reconstruction of hollow organs, e.g. bladder, esophagus, urether, uterus
- A61L 2430/32: for nerve reconstruction
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)

## Abstract

Provided herein are populations of bone marrow mesenchymal stem cells (MSCs) and CD34+ hematopoietic stem/progenitor cells (HSPCs), and methods of isolation and co-administration thereof. In particular, cell populations and methods of co-administration thereof are provided for enhancing tissue (e.g., bladder) regeneration.

## Claims

### Claim 1

A system comprising:
(a) a synthetic elastomeric scaffold for supporting and transplantation of cells,
(b) a cell population of isolated mesenchymal stem cells (MSCs), wherein greater than 90% of the MSCs are CD29+, CD44+, CD73+, CD90+, CD105+, CD166+, CD14−, CD34−, CD45−, CD117−, and CD133−; and
(c) a cell population of hematopoietic stem/progenitor cells (HSPCs), wherein greater than 90% of the HSPCs are CD34+.

### Claim 2 (depends on 1)

The system of claim 1, wherein said scaffold comprises a poly(citrate diol).

### Claim 3 (depends on 1)

The system of claim 1, wherein said isolated MSCs are bone marrow mesenchymal cells.

### Claim 4 (depends on 1)

The system of claim 1, wherein said scaffold is impregnated with said cell population of isolated MSCs and said cell population of HSPCs.

### Claim 5 (depends on 1)

A method of tissue regeneration comprising administering to a subject in need of tissue repair the system of claim 1.

### Claim 6 (depends on 5)

The method of claim 5, wherein said scaffold comprises a poly(diol citrate).

### Claim 7 (depends on 6)

The method of claim 6, wherein the scaffold comprises poly(octandediol citrate).

### Claim 8 (depends on 5)

The method of claim 5, wherein said tissue is bladder tissue.

### Claim 9

An article of manufacture comprising: a cell scaffold configured to be inserted into a subject, wherein said cell scaffold is impregnated with:
(a) a cell population of isolated mesenchymal stem cells (MSCs), wherein greater than 90% of the MSCs are CD29+, CD44+, CD73+, CD90+, CD105+, CD166+, CD14−, CD34−, CD45−, CD117−, and CD133−; and
(b) a cell population of hematopoietic stem/progenitor cells (HSPCs), wherein greater than 90% of the HSPCs are CD34+.

### Claim 10 (depends on 9)

The article of manufacture of claim 9, wherein said cell scaffold is configured to be inserted into a mammalian bladder.