# US 12,005,152 B2 · Microgel composition comprising collagen and method of preparing the same

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- Number: US 12,005,152 B2
- Document ID: US12005152B2
- Status: Granted
- Kind: B2
- Application number: 17/168,780
- Filed: 2021-02-05
- Published: 2021-08-12
- Granted: 2024-06-11
- Earliest claimed: 2020-02-07
- Assignees: Korea Institute of Science and Technology
- Applicants: Korea Institute of Science and Technology
- Inventors: Sang-Heon Kim; Seung Ja Oh; Ha Eun Chung
- Web page: https://patentgrep.com/patent/US12005152B2

## CPC

- A61L 26/0033: {Collagen}
- A61L 26/0023: {Polysaccharides}
- A61L 26/0052: {Mixtures of macromolecular compounds}
- A61L 26/008: {Hydrogels or hydrocolloids}
- A61L 2300/236: Glycosaminoglycans, e.g. heparin, hyaluronic acid, chondroitin
- A61L 2300/252: Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
- A61L 2300/412: Tissue-regenerating or healing or proliferative agents
- A61L 2300/60: characterised by a special physical form
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)

## Abstract

The present disclosure relates to a microgel composition comprising collagen and hyaluronic acid and a method of preparing the same. The microgel according to an embodiment is composed of natural biocompatible substances and is non-toxic and has high mechanical stability or long persistence, and thus can be effectively used in a microgel composition, a three-dimensional scaffold composition, and a tissue-engineering scaffold.

## Claims

### Claim 1

A microgel composition comprising 10% (w/v) of collagen and 2.5% (w/v) to 5% (w/v) of hyaluronic acid,
wherein the composition has a zeta potential of −50 mV to −30 mV,
wherein the composition has a pH of 4 to 11, and
wherein the microgel is in the form of a nonwoven fabric; a woven fabric; a knitted fabric; a fiber bundle; a cylinder including a fiber bundle and a tube into which the fiber bundle is inserted; or a combination of two or more thereof.

### Claim 2 (depends on 1)

The microgel composition of claim 1, wherein the microgel has a size of 10 μm to 250 μm.

### Claim 3 (depends on 1)

The microgel composition of claim 1, wherein the composition is a cell-laden microgel.

### Claim 4 (depends on 1)

The microgel composition of claim 1, wherein the composition does not comprise a chemical crosslinking agent.

### Claim 5

A three-dimensional scaffold comprising 10% (w/v) of collagen and 2.5% (w/v) to 5% (w/v) of hyaluronic acid,
wherein the scaffold has a zeta potential of −50 mV to −30 mV, and
wherein the scaffold is in the form of a nonwoven fabric; a woven fabric; a knitted fabric; a fiber bundle; a cylinder including a fiber bundle and a tube into which the fiber bundle is inserted; or a combination of two or more thereof.

### Claim 6

A scaffold for tissue engineering, comprising 10% (w/v) of collagen and 2.5% (w/v) to 5% (w/v) of hyaluronic acid,
wherein the scaffold has a zeta potential of −50 mV to −30 mV, and
wherein the scaffold is in the form of a nonwoven fabric; a woven fabric; a knitted fabric; a fiber bundle; a cylinder including a fiber bundle and a tube into which the fiber bundle is inserted; or a combination of two or more thereof.

### Claim 7

A method of preparing a microgel composition, the method comprising:
forming an electrostatic collagen-hyaluronic acid complex by reacting 10% (w/v) of collagen and 2.5% (w/v) to 5% (w/v) of hyaluronic acid;
preparing a dispersion of a microgel by breaking the collagen-hyaluronic acid complex; and adjusting pH level of the dispersion to 4-11,
subjecting the dispersion of the microgel to natural sedimentation,
wherein the composition has a zeta potential of −50 mV to −30 mV, and
wherein the microgel is in the form of a nonwoven fabric; a woven fabric; a knitted fabric; a fiber bundle; a cylinder including a fiber bundle and a tube into which the fiber bundle is inserted; or a combination of two or more thereof.

### Claim 8 (depends on 7)

The method of claim 7, wherein the subjecting of the dispersion to natural sedimentation is performed in a temperature range of 20-30° C. for 12-36 hours.