# US 12,005,108 B2 · Lactococcus lactis subspecies lactis isolate WFLU-12 and use thereof

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- Number: US 12,005,108 B2
- Document ID: US12005108B2
- Status: Granted
- Kind: B2
- Application number: 17/643,942
- Filed: 2021-12-13
- Published: 2023-07-27
- Granted: 2024-06-11
- Earliest claimed: 2021-12-13
- Assignees: Pukyong National University Industry-University Cooperation Foundation
- Applicants: Pukyong National University Industry-University Cooperation Foundation
- Inventors: Do-Hyung Kim; Thanh Luan Nguyen; Nam-Eun Kim
- Web page: https://patentgrep.com/patent/US12005108B2

## CPC

- A61K 39/092: {Streptococcus}
- A23K 10/18: of live microorganisms
- A23K 20/195: Antibiotics
- A23K 50/80: for aquatic animals, e.g. fish, crustaceans or molluscs
- A61K 35/744: Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K 39/025: {Enterobacteriales, e.g. Enterobacter}
- A61K 39/09: {Lactobacillales, e.g. aerococcus, enterococcus, lactobacillus, lactococcus}, streptococcus
- C12N 1/20: Bacteria; Culture media therefor
- C12N 1/205: Bacterial isolates
- A23V 2400/231: Lactis
- A61K 2039/106: {Vibrio; Campylobacter; Not used, see subgroups}
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A23K: FODDER
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

The present invention provides a Lactococcus lactis subspecies lactis isolate WFLU-12 with the accession number o KCTC 13180BP, and a use thereof.

## Claims

### Claim 1

A method of providing antibacterial activity to a fish comprising:
administering to the fish a probiotic composition comprising the Lactococcus lactis subspecies lactis isolate WFLU-12 deposited under the accession number KCTC13180BP or a culture thereof.

### Claim 2 (depends on 1)

The method of claim 1, wherein the isolate shows resistance to a temperature of at least 4° C., a pH of 2 to 10, and/or a bile acid.

### Claim 3 (depends on 1)

The method of claim 1, wherein the method provides antibacterial activity against a Gram-negative bacterium of Vibrio anguillarum, V. ichthyoenteri, Aeromonas salmonicida, or Edwardsiella tarda, or a Gram-positive bacterium of Streptococcus iniae, or S. parauberis.

### Claim 4

A method of providing to a fish at least one effect selected from enhancing innate immune response, increasing body weight, increasing body length, enhancing lipid metabolism-related substances and carbon metabolic intermediates, maintaining body circulating metabolites, enhancing body citrulline level, maintaining homeostasis of metabolites, increasing the expression level of N-acetyl methionine, methionine sulfoxide, cysteine sulfonic acid, cysteic acid, increasing the level of taurine and bile acid in intestine, and enhancing the level of B1, B2, B3 and C vitamins in the fish comprising administering to the fish a probiotic composition comprising the Lactococcus lactis subspecies lactis isolate WFLU-12 deposited under the accession number KCTC13180BP or a culture thereof, wherein the increase or the enhancement is when compared to a corresponding fish not administered with the probiotic composition.

### Claim 5

A method of promoting growth of a fish comprising administering to the fish an antibacterial composition comprising the Lactococcus lactis subspecies lactis isolate WFLU-12 deposited under the accession number KCTC13180BP or a culture thereof.

### Claim 6 (depends on 5)

The method of claim 5, wherein the antibacterial composition is a feed additive for fish farming.

### Claim 7

A method of inhibiting the infecting activity of a bacterial pathogen in a fish comprising spraying the fish with an antibacterial composition comprising the Lactococcus lactis subspecies lactis isolate WFLU-12 deposited under the accession number KCTC13180BP or a culture thereof.