# US 12,005,091 B2 · Use of lactic acid bacteria or fermentation product thereof for maintaining or improving gastrointestinal condition

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- Number: US 12,005,091 B2
- Document ID: US12005091B2
- Status: Granted
- Kind: B2
- Application number: 17/726,843
- Filed: 2022-04-22
- Published: 2023-10-05
- Granted: 2024-06-11
- Earliest claimed: 2022-03-29
- Assignees: Glac Biotech Co., Ltd.
- Applicants: Glac Biotech Co., Ltd
- Inventors: Hsieh-Hsun Ho; Wen-Yang Lin; Jui-Fen Chen; Yi-Wei Kuo; Jia-Hung Lin; Chi-Huei Lin; Ching-Wei Chen; Yu-Fen Huang
- Web page: https://patentgrep.com/patent/US12005091B2

## CPC

- A61K 35/747: Lactobacilli, e.g. L. acidophilus or L. brevis
- A61P 31/04: Antibacterial agents
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention discloses a method for maintaining or improving gastrointestinal condition, which includes: administering a lactic acid bacterial composition to a subject in need thereof, wherein the lactic acid bacterial composition comprises: a Lactobacillus paracasei ET-66 strain with a deposition number CGMCC 13514. The present invention also discloses a method for maintaining or improving gastrointestinal condition, which includes: administering a lactic acid bacterial fermentation composition to a subject in need thereof, wherein the lactic acid bacterial fermentation composition comprises: a fermentation product of a Lactobacillus paracasei ET-66 strain.

## Claims

### Claim 1

A method for treating Helicobacter pylori infection, comprising:
administering a lactic acid bacterial fermentation composition to a subject in need thereof, wherein the lactic acid bacterial fermentation composition consists of: a fermentation product of a Lactobacillus paracasei ET-66 strain, a fermentation product of a Lactobacillus salivarius subsp. salicinius AP-32 strain, and a fermentation product of a Lactobacillus plantarum LPL28 strain;
wherein the Lactobacillus paracasei ET-66 strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with a deposition number CGMCC 13514;
wherein the Lactobacillus salivarius subsp. salicinius AP-32 strain is deposited at the China Center for Type Culture Collection (CCTCC) with a deposition number CCTCC M2011127;
wherein the Lactobacillus plantarum LPL28 strain is deposited at the China General Microbiological Culture Collection Center with a deposition number CGMCC 17954;
wherein the lactic acid bacterial fermentation composition is prepared by mixing the Lactobacillus paracasei ET-66 strain, the Lactobacillus salivarius subsp. salicinius AP-32 strain, and the Lactobacillus plantarum LPL28 strain in a fermentation medium at a bacterial count ratio of the Lactobacillus paracasei ET-66 strain to the Lactobacillus salivarius subsp. salicinius AP-32 strain to the Lactobacillus plantarum LPL28 strain as (1 to 8):(1 to 8): 1, cultivating the Lactobacillus paracasei ET-66 strain, the Lactobacillus salivarius subsp. salicinius AP-32 strain, and the Lactobacillus plantarum LPL28 strain in the fermentation medium, and then performing solid-liquid separation on the fermentation medium;
wherein the fermentation product of a Lactobacillus paracasei ET-66 strain is a fermentation liquid free of bacterial strains or a powder of the fermentation liquid free of bacterial strains, the fermentation product of a Lactobacillus salivarius subsp. salicinius AP-32 strain is a fermentation liquid free of bacterial strains or a powder of the fermentation liquid free of bacterial strains, and the fermentation product of a Lactobacillus plantarum LPL28 strain is a fermentation liquid free of bacterial strains or a powder of the fermentation liquid free of bacterial strains.

### Claim 2 (depends on 1)

The method as claimed in claim 1, wherein the fermentation product of a Lactobacillus paracasei ET-66 strain is a powder of the fermentation liquid free of bacterial strains, the fermentation product of a Lactobacillus salivarius subsp. salicinius AP-32 strain is a powder of the fermentation liquid free of bacterial strains, and the fermentation product of a Lactobacillus plantarum LPL28 strain is a powder of the fermentation liquid free of bacterial strains.

### Claim 3 (depends on 1)

The method as claimed in claim 1, wherein the bacterial count ratio of the Lactobacillus paracasei ET-66 strain to the Lactobacillus salivarius subsp. salicinius AP-32 strain to the Lactobacillus plantarum LPL28 strain is 8:1:1.

### Claim 4 (depends on 1)

The method as claimed in claim 1, wherein a temperature for the cultivating is from 25 to 40° C.

### Claim 5 (depends on 1)

The method as claimed in claim 1, wherein a period for the cultivating is from 6 to 12 hours.

### Claim 6 (depends on 1)

The method as claimed in claim 1, wherein a temperature for the cultivating is from 25 to 40° C., and a period for the cultivating is from 6 to 12 hours.

### Claim 7 (depends on 6)

The method as claimed in claim 6, wherein the temperature for the cultivating is 37° C., and the period for the cultivating is 6 hours.

### Claim 8 (depends on 1)

The method as claimed in claim 1, wherein the bacterial count ratio of the Lactobacillus paracasei ET-66 strain to the Lactobacillus salivarius subsp. salicinius AP-32 strain to the Lactobacillus plantarum LPL28 strain is 1:1:1.

### Claim 9 (depends on 1)

The method as claimed in claim 1, wherein the bacterial count ratio of the Lactobacillus paracasei ET-66 strain to the Lactobacillus salivarius subsp. salicinius AP-32 strain to the Lactobacillus plantarum LPL28 strain is 1:8:1.

### Claim 10 (depends on 1)

The method as claimed in claim 1, wherein the solid-liquid separation includes centrifugation or filtration.

### Claim 11 (depends on 1)

The method as claimed in claim 1, wherein the fermentation medium comprises: 1 wt % to 5 wt % of glucose, 2 wt % to 15 wt % of skimmed milk powder, 3 wt % to 10 wt % of peptone, 1 wt % to 5 wt % of yeast extract, 0.05 wt % to 0.2 wt % of potassium hydrogen phosphate, 0 wt % to 0.01 wt % of sodium citrate, 0 wt % to 0.01 wt % of manganese (II) sulfate, 1 wt % to 5 wt % of monosodium glutamate, 0.05 wt % to 0.2 wt % of polysorbate 80, and balance wt % of water.