# US 12,005,087 B2 · Probiotic (Bacillus subtilis) supplementation for improvement of body composition in female athletes

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 12,005,087 B2
- Document ID: US12005087B2
- Status: Granted
- Kind: B2
- Application number: 17/354,208
- Filed: 2021-06-22
- Published: 2021-10-14
- Granted: 2024-06-11
- Earliest claimed: 2017-09-25
- Assignees: Deerland Enzymes, Inc.
- Applicants: Deerland Enzymes, Inc.
- Inventors: John Deaton; Ana Maria Cuentas
- Web page: https://patentgrep.com/patent/US12005087B2

## CPC

- A61K 35/742: Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
- A61K 9/0053: {Mouth and digestive tract, i.e. intraoral and peroral administration}
- A61P 3/04: Anorexiants; Antiobesity agents
- A61K 2035/115: {Probiotics}
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention relates to methods of improving body composition and reducing body fat percentage in an individual. The present invention relates to methods comprising administering to an individual a Bacillus subtilis composition wherein the individual's body fat percentage is reduced.

## Claims

### Claim 1

A method of improving body composition in a female human individual, comprising the step of:
(a) administering orally to the female human individual a composition consisting essentially of an isolated strain of Bacillus subtilis subspecies inaquosorum having accession number NRRL B-67989 in a dose of from about 1×108 CFU per day to about 1×1011 CFU per day for at least 70 days, wherein the body fat percentage of the female human individual is reduced when said individual is submitted to a resistance and/or conditioning training program for at least 70 days.

### Claim 2 (depends on 1)

The method of claim 1, wherein the dose is from about 1×109 CFU per day to about 1×1010 CFU per day.

### Claim 3 (depends on 1)

The method of claim 1, wherein the dose is about 5×109 CFU per day.

### Claim 4 (depends on 1)

The method of claim 1, wherein the administering step is performed for at least 90 days.

### Claim 5 (depends on 4)

The method of claim 4, further comprising the step of:
(b) submitting the female human individual to the resistance training program 3 days per week throughout the entire at least 90 days.

### Claim 6 (depends on 5)

The method of claim 5, further comprising the step of:
(c) submitting the female human individual to the conditioning training program 3 days per week throughout the entire at least 90 days.

### Claim 7 (depends on 1)

The method of claim 1, further comprising the step of:
(b) submitting the female human individual to the resistance training program 3 days per week throughout the entire at least 70 days.

### Claim 8 (depends on 7)

The method of claim 7, further comprising the step of:
(c) submitting the female human individual to the conditioning training program 3 days per week throughout the entire at least 70 days.

### Claim 9 (depends on 1)

The method of claim 1, wherein the body fat percentage of the female human individual is reduced by at least 1%.

### Claim 10 (depends on 1)

The method of claim 1, wherein the body fat percentage of the female human individual is reduced by at least 2%.

### Claim 11 (depends on 1)

The method of claim 1, wherein said female human individual is an adult.