# US 12,002,210 B2 · Analysis software and apparatus for screening early embryo

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- Number: US 12,002,210 B2
- Document ID: US12002210B2
- Status: Granted
- Kind: B2
- Application number: 17/124,729
- Filed: 2020-12-17
- Published: 2021-04-08
- Granted: 2024-06-04
- Earliest claimed: 2018-06-20
- Assignees: JCR Pharmaceuticals Co., Ltd.
- Applicants: JCR Pharmaceuticals Co., Ltd.
- Inventors: Masahide Shiotani; Junko Otsuki
- Web page: https://patentgrep.com/patent/US12002210B2

## CPC

- C12M 41/36: {of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404)}
- G06T 7/0016: {involving temporal comparison}
- A61B 90/20: Surgical microscopes characterised by non-optical aspects
- A61B 90/361: {Image-producing devices, e.g. surgical cameras}
- C12M 21/06: {for in vitro fertilization}
- C12M 41/48: {Automatic or computerized control (automatic analysis G01N35/00)}
- G16H 40/60: for the operation of medical equipment or devices
- G06T 2207/10056: Microscopic image
- G06T 2207/30024: Cell structures in vitro; Tissue sections in vitro
- G06T 2207/30044: Fetus; Embryo
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- G06T: IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D)
- G16H: HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA

## Abstract

Disclosed are a software for analyzing images of a fertilized egg, the software providing a means for executing a process including: (a) a step of measuring the difference in area between the female pronucleus and the male pronucleus from images of a fertilized egg obtained in a period of 1 to 10 hours before the time of occurrence of male and female pronuclear membrane breakdown as a reference; (b) a step of measuring the difference in are between the female pronucleus and the male pronucleus from images of the fertilized egg obtained immediately before the time of occurrence of male and female pronuclear membrane breakdown as the reference; and (c) a step of storing the measured values of the area difference obtained in the step (a) and the area difference obtained in the step (b), to be readable at any time as needed, and an apparatus incorporating this software.

## Claims

### Claim 1

A method, comprising:
capturing an image of a fertilized egg being cultured such that images of the fertilized egg are captured with a predetermined interval; and
causing a computer to perform a method for analyzing an image of the fertilized egg, comprising:
detecting a male pronucleus and a female pronucleus in the images of the fertilized egg captured;
measuring areas of the female pronucleus and the male pronucleus comprising measuring a diameter in a horizontal direction and a diameter in a vertical direction of the female pronucleus and the male pronucleus, and
calculating respective areas of the male pronucleus and the female pronucleus according to the following formula:
area of pronucleus=π×½×diameter in horizontal direction×½×diameter in vertical direction (μm2), by designating the maximum width in the horizontal direction of the pronucleus as the diameter in the horizontal direction, and designating the maximum width in the vertical direction as the diameter in the vertical direction,
wherein the areas of the female pronucleus and the male pronucleus are measured over time with intervals of every 5 minutes to every 1 hour,
measuring a first area difference between the female pronucleus and the male pronucleus from an image of a fertilized egg captured at a point of time in a period from 1 hour before to 10 hours before a time of occurrence of male and female pronuclear membrane breakdown;
measuring a second area difference between the female pronucleus and the male pronucleus from an image of a fertilized egg captured at a point of time in a period from immediately before to 20 minutes before the time of occurrence of the male and female pronuclear membrane breakdown;
selecting a fertilized egg to be transplanted into the uterine cavity based on measured values of the first area difference and the second area difference, wherein the selecting comprises selecting a fertilized egg that satisfies that the second area difference is less than 40 μm2, and
transplanting the fertilized egg into the uterine cavity.

### Claim 2 (depends on 1)

The analytical method according to claim 1, wherein the selecting comprises selecting the fertilized egg that further satisfies that the second area difference is smaller than a difference in area between the female pronucleus and the male pronucleus measured at a point of time in a period from 7 hours before to 10 hours before the time of occurrence of the male and female pronuclear membrane breakdown.

### Claim 3 (depends on 1)

The analytical method according to claim 1, wherein the selecting further comprises selecting the fertilized egg that further satisfies that a difference in area measured at a point of time in a period from 7.5 hours before to 8.5 hours before the time of occurrence of the male and female pronuclear membrane breakdown is 1.3 times or greater than the second area difference.

### Claim 4 (depends on 1)

The analytical method according to claim 1, wherein the selecting further comprises selecting the fertilized egg that further satisfies that an area ratio of the female pronucleus and the male pronucleus measured at any point of time in a period from immediately after to 8 hours after a time of occurrence of pronuclear formation is greater than 1.

### Claim 5 (depends on 2)

The analytical method according to claim 2, wherein the selecting further comprises selecting the fertilized egg that further satisfies that an area ratio of the female pronucleus and the male pronucleus measured at any point of time in a period from immediately after to 8 hours after a time of occurrence of pronuclear formation is greater than 1.

### Claim 6 (depends on 1)

The analytical method according to claim 1, wherein the capturing of the image is conducted at an interval of 1 minute to 5 minutes between each capturing.

### Claim 7 (depends on 2)

The analytical method according to claim 2, wherein the capturing of the image is conducted at an interval of 1 minute to 5 minutes between each capturing.

### Claim 8 (depends on 3)

The analytical method according to claim 3, wherein the capturing of the image is conducted at an interval of 1 minute to 5 minutes between each capturing.

### Claim 9 (depends on 4)

The analytical method according to claim 4, wherein the capturing of the image is conducted at an interval of 1 minute to 5 minutes between each capturing.

### Claim 10 (depends on 5)

The analytical method according to claim 5, wherein the capturing of the image is conducted at an interval of 1 minute to 5 minutes between each capturing.

### Claim 11

A method, comprising:
subjecting a patient to transplantation of freeze-thawed blastocysts under hormone replacement therapy (HRT) with estrogen and progesterone, comprising
in a pretreatment cycle, initiating application of gonadotropin-releasing hormone (GnRH) agonist on day 7 of the hyperthermic phase in the cycle before treatment, and from day 3 of menstruation and thereafter, subjecting the patient to daily stimulation of ovaries with follicle-stimulating hormone, until a diameter of the largest subordinate follicle reached a predetermined value, and triggering ovulation,
intramuscularly injecting human chorionic gonadotropin (hCG), and transvaginally collecting ova under ultrasonographic guidance after the injection, and measuring follicles by ultrasonic scanning,
fertilizing the collected ova by an insemination method or an intracytoplasmic sperm injection method and culturing fertilized eggs in a medium 1 to obtain early embryos,
capturing an image of a fertilized egg being cultured such that images of the fertilized egg are captured with a predetermined interval;
detecting a male pronucleus and a female pronucleus in the images of the fertilized egg captured;
measuring a diameter in a horizontal direction and a diameter in a vertical direction of the male pronucleus and the female pronucleus;
calculating respective areas of the male pronucleus and the female pronucleus according to the following formula:
area of pronucleus=π×½×diameter in horizontal direction×½×diameter in vertical direction (μm2), by designating the maximum width in the horizontal direction of the pronucleus as the diameter in the horizontal direction, and designating the maximum width in the vertical direction as the diameter in the vertical direction,
wherein the areas of the female pronucleus and the male pronucleus are measured over time with intervals of every 5 minutes to every 1 hour,
calculating a first area difference between the respective areas of the female pronucleus and the male pronucleus from an image of the fertilized egg captured at a point of time in a period from 1 hour before to 10 hours before a time of occurrence of male and female pronuclear membrane breakdown;
calculating a second area difference between the respective areas of the female pronucleus and the male pronucleus from an image of the fertilized egg captured at a point of time in a period from immediately before to 20 minutes before the time of occurrence of the male and female pronuclear membrane breakdown; and
selecting a fertilized egg for transplantation based on calculated values of the first area difference and the second area difference, wherein the selecting comprises selecting the fertilized egg that satisfies that the second area difference is less than 40 μm2,
culturing at least one of the obtained early embryos obtained from the selected fertilized egg in a medium 2 on a culture plate in an incubator, and obtaining blastocysts, and
transplanting the blastocysts obtained in the culturing of the selected fertilized egg into the uterine cavity, and determining a subsequent birth status of the transplanted patients.