Diagnostic kits and methods configured to rapidly and non-invasively determine physiologic levels of galactose-deficient IgA1 (Gd-IgA1) in the subject of IgA nephropathy
AssigneeNational Defense Medical Center & Academia Sinica
InventorsAnn Chen, Shuk-Man Ka, Shih-Hsiung Wu
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Abstract
The present invention provides a lectin from Crenomytilus grayanus or Mytilus trossulus as diagnostic reagents for IgA nephropathy. The present invention also provides a diagnostic kit for detecting Galactose-deficient IgA1 (Gd-IgA1), comprising RussiaSea-001 (also called as CGL) isolated from Crenomytilus grayanus or RussiaSea-002 (also called as MTL) isolated from Mytilus trossulus. The present invention further provides a method for detecting Gd-IgA1 using RussiaSea-001 or RussiaSea-002.
Claims6
A method for detecting galactose-deficient IgA1 (Gd-IgA1), comprising
- (a) providing a sample from a subject,
- (b) providing a kit of diagnosing galactose-deficient IgA1 (Gd-IgA1), comprising a lectin from Crenomytilus grayanus, wherein the lectin is RussiaSea-001 (Crenomytilus grayanus lectin/CGL), wherein the RussiaSea-001 is SEQ ID NO:
- 1, wherein the SEQ ID NO:
- 1 has a specificity to N-acetyl-2-deoxy-2-amino-galactose (GalNAc/Gal), wherein the kit is applied to diagnosing a subject with galactose-deficient IgA1, wherein the subject is selected from IgA nephropathy (IgAN) patient, and lupus nephritis (LN) patients, and
- (c) treating the sample with the kit resulting in the specific binding capability between lectin and Gd-IgA1.
A kit for diagnosing galactose-deficient IgA1 (Gd-IgA1), comprising a lectin isolated from Mytilus trossulus, wherein the lectin is RussiaSea-002 (Mytilus trossulus lectin/MTL), wherein the RussiaSea-002 is SEQ ID NO:
- 2, wherein the SEQ ID NO:
- 2 has a specificity to N-acetyl-2-deoxy-2-amino-galactose (GalNAc/Gal), wherein the kit is applied to diagnosing a subject with galactose-deficient IgA1, wherein the subject is selected from IgA nephropathy (IgAN) patient, and lupus nephritis (LN) patients.
Dependencies are inferred from the claim text.