# US 12,000,837 B1 · Granule for detecting protein in pet urine and method for preparing the same

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- Number: US 12,000,837 B1
- Document ID: US12000837B1
- Status: Granted
- Kind: B1
- Application number: 18/516,927
- Filed: 2023-11-21
- Granted: 2024-06-04
- Earliest claimed: 2023-08-02
- Assignees: Shandong Ruida Silica Gel Co., Ltd.
- Applicants: Shandong Ruida Silica Gel Co., Ltd.
- Inventors: Qinbin Sun
- Web page: https://patentgrep.com/patent/US12000837B1

## CPC

- G01N 33/68: involving proteins, peptides or amino acids {(involving lipoproteins G01N33/92)}
- G01N 33/6839: {involving dyes, e.g. Coomassie blue, bromcresol green}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A granule for detecting protein in pet urine comprises 60-80 parts by weight of silicone particles, 40-50 parts by weight of a filler, 10-20 parts by weight of an adhesive, 1-3 parts by weight of a bacteriostatic agent, 3-5 parts by weight of a deodorant, and 5-10 parts by weight of an tetrabromophenol blue indicator.

## Claims

### Claim 1

A granule for detecting protein in pet urine, the granule comprising: 60-80 parts by weight of silicone particles, 40-50 parts by weight of a filler, 10-20 parts by weight of an adhesive, 1-3 parts by weight of a bacteriostatic agent, 3-5 parts by weight of a deodorant, and 5-10 parts by weight of a tetrabromophenol blue indicator.

### Claim 2 (depends on 1)

The granule of claim 1, wherein the silicone particles are modified silicone particles, and are prepared as follows:
a) ultrasonically cleaning the silicone particles in 30-40° C. water for 10-20 min, and further ultrasonically cleaning in industrial alcohol for 3-5 min, and drying;
b) soaking the silicone particles treated in a) in N-(2-aminoethyl)-3-aminopropyltrimethoxysilane for 5-10 min, and drying at 50-60° C. in a drying oven; and
c) soaking the silicone particles treated in b) in a mixed solution comprising polyethylene pyrrolidone, ethyl cellulose, N, N-dimethylformamide, ethyl acetate, and anhydrous ethanol for 10-15 min, and drying at 30-40° C., to yield modified silicone particles.

### Claim 3 (depends on 2)

The granule of claim 2, wherein in c), a mass ratio of polyethylene pyrrolidone to ethyl cellulose to N, N-dimethylformamide to ethyl acetate to anhydrous ethanol is 1:0.2:5:1:10.

### Claim 4 (depends on 2)

The granule of claim 2, wherein a particle size of the modified silicone particles is 1-2 mm.

### Claim 5 (depends on 1)

The granule of claim 1, wherein the filler is modified starch, polyester fiber, acetate fiber, nylon fiber, microcrystalline cellulose, or a mixture thereof.

### Claim 6 (depends on 1)

The granule of claim 1, wherein the adhesive is sodium carboxymethyl cellulose, pectin, guar gum, sodium alginate, xanthan gum, or a mixture thereof.

### Claim 7 (depends on 1)

The granule of claim 1, wherein the bacteriostatic agent is polyhexamethylene biguanide hydrochloride, sodium benzoate, potassium sorbate, or a mixture thereof.

### Claim 8 (depends on 1)

The granule of claim 1, wherein the deodorant is mugwort leaf, grapefruit peel, mulberry leaf, or a mixture thereof.

### Claim 9 (depends on 1)

A method for preparing the granule for detecting protein in pet urine of claim 1, the method comprising:
1) Crushing the silicone particles, the filler, the adhesive, the bacteriostatic agent, and the deodorant to particle size of 1-2 mm, and drying;
2) Dissolving tetrabromophenol blue in 0.1 mol/L NaOH solution, and diluting the NaOH solution containing dissolved tetrabromophenol blue with distilled water until a concentration of tetrabromophenol blue is 0.06% in the NaOH solution, whereby obtaining a tetrabromophenol blue indicator solution;
3) Soaking crushed silicone particles in the tetrabromophenol blue indicator solution obtained in 2) for 30-40 min, and drying; and
4) Mixing the crushed silicone particles treated in 3) and the filler, the adhesive, the bacteriostatic agent, and the deodorant treated in 1), feeding a resulting mixture to a tablet press machine for granulation, to yield flaky granules.

### Claim 10 (depends on 9)

The method of claim 9, wherein the complete flaky granules have a diameter of 30 mm, and a thickness of 4 mm.