# US 12,000,828 B2 · Immunodetection chip, immunodetection device and using method

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- Number: US 12,000,828 B2
- Document ID: US12000828B2
- Status: Granted
- Kind: B2
- Application number: 16/758,163
- Filed: 2019-03-25
- Published: 2021-07-15
- Granted: 2024-06-04
- Earliest claimed: 2019-03-25
- Assignees: Boe Technology Group Co., Ltd.
- Applicants: BOE Technology Group Co., Ltd.
- Inventors: Hao Tang; Quanguo Zhou; Jiuyang Cheng; Lijia Zhou; Zhidong Wang; Yancheng Lu; Ronghua Lan
- Web page: https://patentgrep.com/patent/US12000828B2

## CPC

- G01N 33/54306: {Solid-phase reaction mechanisms}
- G01N 33/54326: {Magnetic particles}
- G01N 27/745: {for detecting magnetic beads used in biochemical assays (concerning the assays G01N33/54326; sensors therefor G01R33/1269; automatic analysers therefor G01N35/0098)}
- G01N 33/54386: {Analytical elements}
- G01N 2470/04: Sandwich assay format
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

An embodiment of the disclosure provides an immunodetection chip, an immunodetection device and a using method. The immunodetection chip includes a substrate and a cover plate. The substrate is disposed opposite to the cover plate to form a detection chamber. One side, facing the cover plate, of the substrate is fixedly provided with substrate antibodies. An inside wall of the detection chamber is provided with a detection member. The detection member is configured to output a corresponding electrical signal while adsorbing biological magnetic beads. The substrate antibodies match with target antigens.

## Claims

### Claim 1

An immunodetection chip, comprising:
a substrate, and
a cover plate;
wherein the substrate is disposed opposite to the cover plate to form a detection chamber;
one side, facing the cover plate, of the substrate is fixedly provided with substrate antibodies, and an inside wall of the detection chamber is provided with a detection member;
wherein the detection member is located between the substrate and the substrate antibodies, and a biological modification layer is disposed between the substrate antibodies and the detection member;
wherein the biological modification layer is made of nanogold and directly in contact with the substrate antibodies and the detection member respectively;
wherein the detection member is configured to output a corresponding electrical signal while adsorbing biological magnetic beads via the biological modification layer;
wherein the substrate antibodies match with target antigens.

### Claim 2 (depends on 1)

An immunodetection device, wherein the immunodetection device comprises the immunodetection chip of claim 1.

### Claim 3 (depends on 2)

The immunodetection device of claim 2, wherein the immunodetection device further comprises biological magnetic beads, wherein the biological magnetic beads each comprises a magnetic bead and antibodies enveloping the magnetic bead, and the biological magnetic beads are added to the detection chamber of the immunodetection chip while being used to detect antigens in liquid to be detected.

### Claim 4 (depends on 2)

The immunodetection device of claim 2, wherein the immunodetection device further comprises a magnetic field generation member, wherein the magnetic field generation member applies a magnetic field to the immunodetection chip while being used to detect antigens in liquid to be detected.

### Claim 5 (depends on 4)

The immunodetection device of claim 4, wherein the magnetic field generation member is located on one side, facing away from the substrate, of the cover plate.

### Claim 6 (depends on 4)

The immunodetection device of claim 4, wherein the magnetic field generation member is located on one side, facing away from the cover plate, of the substrate.