# US 12,000,783 B2 · Device for detecting substance being measured > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,000,783 B2 - Document ID: US12000783B2 - Status: Granted - Kind: B2 - Application number: 17/626,124 - Filed: 2020-07-10 - Published: 2022-09-01 - Granted: 2024-06-04 - Earliest claimed: 2019-07-11 - Assignees: Citizen Watch Co., Ltd. - Applicants: Citizen Watch Co., Ltd. - Inventors: Kana Wada; Takaaki Nozaki - Web page: https://patentgrep.com/patent/US12000783B2 ## CPC - G01N 21/64: Fluorescence; Phosphorescence - B03C 1/01: by addition of magnetic adjuvants - B03C 1/02: acting directly on the substance being separated - B03C 1/0332: {using permanent magnets} - B03C 1/0335: {using coils} - B03C 1/288: {disposed at the outer circumference of a recipient} - B03C 1/30: Combinations with other devices, not otherwise provided for - G01N 33/483: Physical analysis of biological material - G01N 33/543: with an insoluble carrier for immobilising immunochemicals - G01R 33/0017: {Means for compensating offset magnetic fields or the magnetic flux to be measured; Means for generating calibration magnetic fields} - B03C 2201/18: Magnetic separation whereby the particles are suspended in a liquid - B03C 2201/26: for use in medical or biological applications - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - B03C: MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS (separating isotopes B01D59/00; combinations of magnetic or electrostatic separation with separation of solids by other means B03B, B07B) - G01R: MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES (indicating correct tuning of resonant circuits H03J3/12) ## Abstract A detection device of a substance to be measured according to an embodiment of the present disclosure is intended to conveniently detect a bio-related substance such as a bacteria or a fungus. The detection device according to an embodiment of the present disclosure includes, a container that contains solution and a composite particle combining a substance to be measured and a magnetic labeling substance, a magnetic field applying unit that applies a magnetic field to a predetermined region so as to collect the composite particles, wherein spatial light is incident to the predetermined region other than lower region of the container, an imaging unit for imaging the composite particles collected in the predetermined region where the spatial light is incident, a detection unit that detects the composite particles based on the image captured by the imaging unit. ## Claims ### Claim 1 A detection device comprising: a container that contains solution and a composite particle combining a substance to be measured and a magnetic labeling substance; a magnetic field applying unit that applies a magnetic field to a predetermined region so as to collect the composite particle, wherein spatial light is incident to the predetermined region other than lower region of the container; an imaging unit that captures an image of the composite particle collected in the predetermined region where the spatial light is incident; and a detecting unit that detects the composite particle based on the image captured by the imaging unit wherein the imaging unit is disposed at a position facing the container across the magnetic field applying unit, and wherein the magnetic field applying unit moves to a position relative to the container where the magnetic field applying unit does not interfere capturing the image by the imaging unit when the imaging unit captures the image. ### Claim 2 (depends on 1) The detection device according to claim 1, wherein the magnetic field applying unit includes: a first plane member opposed to the upper surface of the solution; and a second plane member facing the imaging unit, and wherein an area of the first plane member is smaller than an area of the second plane member. ### Claim 3 (depends on 1) The detection device according to claim 1, wherein the magnetic field applying unit has a shape in which the cross-sectional area increases continuously or stepwise, as it proceeds upward from the lower end of the magnetic field applying unit. ### Claim 4 (depends on 1) The detection device according to claim 1, wherein the magnetic field applying unit applies a magnetic field to the solution so that the composite particle is distributed in an imaging region where the imaging unit captures an image. ### Claim 5 (depends on 1) The detection device according to claim 1, wherein the imaging region captured by the imaging unit is a part of the region occupied by the container, and wherein the closest portion of the upper surface side of the solution in the magnetic field applying unit has a size included in the imaging region. ### Claim 6 (depends on 2) The detection device according to claim 2, wherein the magnetic field applying unit has a plurality of laminated magnets. ### Claim 7 (depends on 2) The detection device according to claim 2, wherein the magnetic field applying unit has an integrally molded magnet. ### Claim 8 (depends on 6) The detection device according to claim 6, wherein the plurality of magnets have a cylindrical shape or a prismatic shape. ### Claim 9 (depends on 1) The detection device according to claim 1, the magnetic field applying unit is relatively movable with respect to the container between a position where a closest portion of an upper surface side of the solution in the magnetic field applying unit opposed to the upper surface of the solution and a position where the magnetic field applying unit does not interfere with the imaging unit capturing an image of the upper surface of the solution. ### Claim 10 (depends on 9) The detection device according to claim 9, further comprising a control unit for controlling the magnetic field applying unit so that the magnetic field applying unit moves relative to the container to the position where the magnetic field applying unit does not interfere with capturing an image by the imaging unit, after the closest portion of the magnetic field applying unit moves vertically upward to a position where the influence of the magnetic field does not affect the composite particles from a position facing the upper surface of the solution. ### Claim 11 A detection device comprising: a container that contains solution and a composite particle combining a substance to be measured and a magnetic labeling substance; a magnetic field applying unit that applies a magnetic field to a predetermined region so as to collect the composite particle, wherein spatial light is incident to the predetermined region other than lower region of the container; an imaging unit that captures an image of the composite particle collected in the predetermined region where the spatial light is incident; and a detecting unit that detects the composite particle based on the image captured by the imaging unit wherein the magnetic field applying unit is disposed above the container, and wherein the imaging unit is disposed below the container. ### Claim 12 A detection device comprising: a container that contains solution and a composite particle combining a substance to be measured and a magnetic labeling substance; a magnetic field applying unit that applies a magnetic field to a predetermined region so as to collect the composite particle, wherein spatial light is incident to the predetermined region other than lower region of the container; an imaging unit that captures an image of the composite particle collected in the predetermined region where the spatial light is incident; and a detecting unit that detects the composite particle based on the image captured by the imaging unit wherein the magnetic field applying unit has a first coil, and wherein the imaging unit is disposed at a position facing the container across the first coil, so as to capture an image of the inside of the container through the inside of the first coil. ### Claim 13 (depends on 12) The detection device according to claim 12, wherein the magnetic field applying unit further includes a second coil, and wherein the second coil is disposed at a position such that a magnetic field can be applied to a position different from the position where the magnetic field is applied by the first coil. ### Claim 14 (depends on 12) The detection device according to claim 12, wherein the magnetic field applying unit further includes a second coil, and further comprising a control unit configuring to stop applying the magnetic field by the first coil after a first predetermined time has elapsed since start of the applying the magnetic field by the first coil, start applying the magnetic field by the second coil, and make the imaging unit capture an image after the second predetermined time has elapsed from the start of the applying the magnetic field by the second coil.