# US 12,000,771 B2 · Light irradiation apparatus > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,000,771 B2 - Document ID: US12000771B2 - Status: Granted - Kind: B2 - Application number: 17/630,142 - Filed: 2020-07-27 - Published: 2022-09-01 - Granted: 2024-06-04 - Earliest claimed: 2019-07-25 - Assignees: Seoul Viosys Co., Ltd. - Applicants: Seoul Viosys Co., Ltd. - Inventors: Hee Ho Bae; Yeong Min Yoon; A Young Lee - Web page: https://patentgrep.com/patent/US12000771B2 ## CPC - G01N 15/1429: Signal processing - G01N 1/30: Staining; Impregnating {; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis} - G01N 15/00: Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials - G01N 15/1436: {the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell} - G01N 21/01: Arrangements or apparatus for facilitating the optical investigation - G01N 21/33: using ultraviolet light (G01N21/39 takes precedence) - G01N 27/447: using electrophoresis - G01N 27/44721: {by optical means} - G01N 33/49: Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)} - G01N 33/491: {by separating the blood components (G01N15/05 takes precedence)} - C12M 41/46: {of cellular or enzymatic activity or functionality, e.g. cell viability} - G01N 15/01: specially adapted for biological cells, e.g. blood cells (investigating sedimentation of particle suspensions in blood G01N15/05) - G01N 21/6428: {Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53)} - G01N 21/6486: {Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence)} - G01N 2021/0112: {Apparatus in one mechanical, optical or electronic block} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract A light irradiation device including an injection unit to inject a sample, and a light source to apply light to the sample to identify an abnormal cell in the sample, the light source including a substrate and a light emitter including a light emitting diode, in which the light emitted is to cut a genetic material in the sample into sections of different sizes, and to deform the genetic material to different degrees, such that a determination of an abnormality of the sample is based on the degree of deformation, and an irradiation amount or intensity of the light is at an intensity in which a cytotoxicity value of the sample is greater than or equal to a predetermined value, and a ratio of a degree of deformation of the genetic material of the normal sample to that of the abnormal sample is set to be a minimum value. ## Claims ### Claim 1 A light irradiation device comprising: an injection unit configured to inject a sample; and a light source configured to apply light to the sample to identify an abnormal cell in the sample, the light source including: a substrate; and a light emitter disposed on the substrate and comprising a light emitting diode, wherein the light emitted from the light emitter is configured to cut a genetic material in the sample into sections of different sizes depending on a normal sample or an abnormal sample, and to deform the genetic material to different degrees, such that a determination of an abnormality of the sample is based on the degree of deformation of the genetic material, and wherein an irradiation amount or an irradiation intensity of the light emitted from the light emitter is configured to be provided at an intensity in which a cytotoxicity value of the sample is greater than or equal to a predetermined value, and in which a ratio of a degree of deformation of the genetic material of the normal sample to a degree of deformation of the genetic material of the abnormal sample is set to be a minimum value outside an error range. ### Claim 2 (depends on 1) The light irradiation device of claim 1, wherein the light emitted from the light emitter is in a wavelength band that generates reactive oxygen species, such that the light transmitted into the sample stimulates a photosensitive material in the sample. ### Claim 3 (depends on 1) The light irradiation device of claim 1, further comprising: a reaction unit configured to inject a dyeing material into the sample to cause reaction between the dyeing material and the sample; and an analysis unit configured to analyze the sample. ### Claim 4 (depends on 3) The light irradiation device of claim 3, further comprising a pre-processing unit configured to process the sample before providing the sample to the reaction unit. ### Claim 5 (depends on 4) The light irradiation device of claim 4, wherein the sample comprises blood. ### Claim 6 (depends on 5) The light irradiation device of claim 5, wherein the sample comprises leukocyte, and the pre-processing unit is configured to separate the leukocyte from whole blood. ### Claim 7 (depends on 3) The light irradiation device of claim 3, wherein the analysis unit is configured to be implemented in a Comet assay. ### Claim 8 (depends on 7) The light irradiation device of claim 7, wherein the analysis unit includes: a data providing unit configured to provide a table quantifying a degree of damage of the genetic material according to a length of a tail in an electrophoresis result; a comparison unit configured to compare lengths of tails of a normal cell and an abnormal cell with the length of the tail stored in the data providing unit; and a determination unit configured to determine whether cancer occurs based on the comparison result of the comparison unit. ### Claim 9 (depends on 8) The light irradiation device of claim 8, wherein the data providing unit has a table corresponding to either a normal or an abnormal state according to the length of the tail. ### Claim 10 (depends on 1) The light irradiation device of claim 1, wherein the light emitted from the light emitter includes at least a portion of light in a blue wavelength band to an ultraviolet wavelength band. ### Claim 11 (depends on 10) The light irradiation device of claim 10, wherein the irradiation amount of the light is configured to be set within a limit in which cell viability of the normal sample is 75% or more. ### Claim 12 (depends on 10) The light irradiation device of claim 10, wherein the irradiation amount of the light is configured to be about 5 J/cm2 or less, and the light is configured to be irradiated with an irradiation time of about 15 minutes or less. ### Claim 13 (depends on 10) The light irradiation device of claim 10, wherein the light is configured to be irradiated with a quantity of light of about 1.5 mW/cm2 to about 100 mW/cm2. ### Claim 14 (depends on 1) The light irradiation device of claim 1, wherein the light corresponds to at least one of light in UVA, UVB, and UVC wavelength bands and light in the blue wavelength band. ### Claim 15 (depends on 1) The light irradiation device of claim 1, wherein the light source includes at least two light emitters configured to emit light having different wavelengths. ### Claim 16 A light irradiation device comprising: an injection unit configured to inject a sample; and a light source configured to apply light to the sample, wherein the light source includes: a light emitter configured to emit light to the sample to identify an abnormal cell in the sample; and a substrate on which the light emitter is disposed to dissipate heat generated from the light emitter, wherein the light emitted from the light emitter is configured to cut a DNA or RNA in the sample into sections of different sizes depending on a normal sample or an abnormal sample, and to deform the DNA or RNA to different degrees, such that a determination of the abnormality of the sample is based on the degree of deformation of the DNA or RNA, and wherein an irradiation amount or irradiation intensity of the light is configured to be set within a limit in which cell viability of the normal sample is 75% or more. ### Claim 17 (depends on 16) The light irradiation device of claim 16, wherein the light emitted from the light emitter includes at least a portion of light in a blue wavelength band to an ultraviolet wavelength band. ### Claim 18 (depends on 16) The light irradiation device of the claim 16, wherein the irradiation amount of the light is configured to be about 5 J/cm2 or less, and the light is configured to be irradiated with an irradiation time of about 15 minutes or less. ### Claim 19 (depends on 16) The light irradiation device of claim 16, wherein the light is configured to be irradiated with a quantity of light of about 1.5 mW/cm2 to about 100 mW/cm2. ### Claim 20 (depends on 16) The light irradiation device of claim 16, wherein the sample comprises blood.